A Proof of the Low Speed Centrifugation Concept in Rodents: New Perspectives for In Vivo Research

Tissue Eng Part C Methods. 2018 Nov;24(11):659-670. doi: 10.1089/ten.TEC.2018.0236.

Abstract

This study evaluated for the first time the composition and bioactivity of platelet-rich fibrin (PRF) produced from small animal blood by reducing the initial blood volume needed for the preparation of PRF from 10 to 3 mL. The results showed that different preparation protocols of PRF produced using 3 mL of animal blood exhibit the same composition, properties, and bioactivity as PRF prepared using 10 mL human blood.

Keywords: LSCC; PRF; animal model; reduction; regeneration.

MeSH terms

  • Animals
  • Biomarkers / metabolism*
  • Blood Platelets / cytology
  • Blood Platelets / metabolism*
  • Cells, Cultured
  • Centrifugation / methods*
  • Female
  • Leukocytes / cytology
  • Leukocytes / metabolism*
  • Lymphocytes / cytology
  • Lymphocytes / metabolism*
  • Platelet-Rich Fibrin / metabolism*
  • Rats
  • Rats, Wistar

Substances

  • Biomarkers