Characterization of inv(3) cell line OCI-AML-20 with stroma-dependent CD34 expression

Exp Hematol. 2019 Jan:69:27-36. doi: 10.1016/j.exphem.2018.10.006. Epub 2018 Oct 22.

Abstract

Acute myeloid leukemia (AML) is a complex, heterogeneous disease with variable outcomes following curative intent chemotherapy. AML with inv(3) is a genetic subgroup characterized by a very low response rate to current induction type chemotherapy and thus has among the worst long-term survivorship of the AMLs. Here, we describe OCI-AML-20, a new AML cell line with inv(3) and deletion of chromosome 7; the latter is a common co-occurrence in inv(3) AML. In OCI-AML-20, CD34 expression is maintained and required for repopulation in vitro and in vivo. CD34 expression in OCI-AML-20 shows dependence on the co-culture with stromal cells. Transcriptome analysis indicates that the OCI-AML-20 clusters with other AML patient data sets that have poor prognosis, as well as other AML cell lines, including another inv(3) line, MUTZ-3. OCI-AML-20 is a new cell line resource for studying the biology of inv(3) AML that can be used to identify potential therapies for this poor outcome disease.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Antigens, CD34 / biosynthesis*
  • Antigens, CD34 / genetics
  • Cell Line, Tumor* / metabolism
  • Cell Line, Tumor* / pathology
  • Chromosome Deletion*
  • Chromosome Inversion*
  • Chromosomes, Human, Pair 3 / genetics*
  • Chromosomes, Human, Pair 3 / metabolism
  • Chromosomes, Human, Pair 7 / genetics*
  • Chromosomes, Human, Pair 7 / metabolism
  • Coculture Techniques
  • Gene Expression Regulation, Leukemic*
  • Humans
  • Leukemia, Myeloid, Acute* / genetics
  • Leukemia, Myeloid, Acute* / metabolism
  • Leukemia, Myeloid, Acute* / pathology
  • Male
  • Neoplasm Proteins / biosynthesis*
  • Neoplasm Proteins / genetics
  • Stromal Cells / metabolism
  • Stromal Cells / pathology

Substances

  • Antigens, CD34
  • Neoplasm Proteins