Preparation, characterization and in vitro release study of drug-loaded sodium carboxy-methylcellulose/chitosan composite sponge

Baoyi Cai; Tian Zhong; Peizhou Chen; Jia Fu; Yuanbao Jin; Yinglei Liu; Ran Huang; Lianjiang Tan

doi:10.1371/journal.pone.0206275

Preparation, characterization and in vitro release study of drug-loaded sodium carboxy-methylcellulose/chitosan composite sponge

PLoS One. 2018 Oct 22;13(10):e0206275. doi: 10.1371/journal.pone.0206275. eCollection 2018.

Authors

Baoyi Cai¹, Tian Zhong^{1

2}, Peizhou Chen¹, Jia Fu³, Yuanbao Jin¹, Yinglei Liu¹, Ran Huang^{2

4}, Lianjiang Tan²

Affiliations

¹ School of Pharmacy and Food Science, Zhuhai College of Jilin University, Zhuhai, Guangdong, China.
² Department of Materials Technology and Engineering, Research Institute of Zhejiang University-Taizhou, Taizhou, Zhejiang, China.
³ School of Health, Zhuhai College of Jilin University, Zhuhai, Guangdong, China.
⁴ State Key Laboratory of Microbial Metabolism and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.

Abstract

A sodium carboxy-methylcellulose (CMC)/chitosan (CS) composite sponge as drug carrier was prepared, and its structure and functions were investigated. Samples with different CMC/chitosan ratios and under different pH conditions were synthesized via a freeze-drying method. The microstructure of the dried sponges was analyzed by Scanning Electron Microscope (SEM). Molecule interactions between polymers were confirmed by Fourier transform infrared (FTIR) spectra and Thermal gravimetric analyze (TGA). The swelling degree, weight loss, in vitro drug release behavior and antibacterial property of the sponges were determined as well. The results showed that the CMC/chitosan ratio and the pH value significantly affected the appearance of the blending solution and the microstructure of the final product, and also affected the sponge's degradation behavior, drug-loading capacity and the antibacterial activity. Gentamicin (GEN) as a hydrophilic model drug was remarkably superior to the other two hydrophobic drugs, ibuprofen (IBU) and roxithromycin (ROX), with respect to in vitro releasing. Moreover, higher CMC content and lower pH value of the sponge were confirmed to lead a larger loading for GEN. The bacteriostatic experiment showed a strong antimicrobial ability of GEN-loaded sponges on inhibiting Escherichia coli.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bandages
Carboxymethylcellulose Sodium / chemistry*
Chitosan / chemistry*
Drug Carriers / chemistry*
Drug Liberation
Escherichia coli / drug effects
Freeze Drying
Gentamicins / chemistry
Gentamicins / metabolism
Gentamicins / pharmacology
Hydrogen-Ion Concentration
Hydrophobic and Hydrophilic Interactions
Ibuprofen / chemistry
Ibuprofen / metabolism
Microscopy, Electron, Scanning
Pharmaceutical Preparations / chemistry*
Pharmaceutical Preparations / metabolism
Roxithromycin / chemistry
Roxithromycin / metabolism
Spectroscopy, Fourier Transform Infrared
Viscosity

Substances

Drug Carriers
Gentamicins
Pharmaceutical Preparations
Roxithromycin
Chitosan
Carboxymethylcellulose Sodium
Ibuprofen

Grants and funding

This work was supported by: National Natural Science Foundation of China (Grant No. 11505110) to RH (URL: http://www.nsfc.gov.cn/); China Postdoctoral Science Foundation (Grant No. 2016M591666) to RH (URL: http://jj.chinapostdoctor.org.cn/); Shanghai Pujiang Talent Grant (Grant No. 16PJ1431900) to RH (URL: N/A); Innovation Project by the Education Bureau of Guangdong Province (Grant No. 2016KTSCX175) to TZ (URL: N/A); Taizhou Municipal Science and Technology Program (Grant No. 1701GY15 to RH and 2017CLS01 to LT). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.