Absence of toll-like receptor 9 Pro99Leu polymorphism in cervical cancer

Alex Chauhan; Nilesh Pandey; Nitin Raithatha; Purvi Patel; Ajesh Desai; Neeraj Jain

doi:10.12688/f1000research.14840.2

Absence of toll-like receptor 9 Pro99Leu polymorphism in cervical cancer

F1000Res. 2018 May 17:7:606. doi: 10.12688/f1000research.14840.2. eCollection 2018.

Authors

Alex Chauhan¹, Nilesh Pandey¹, Nitin Raithatha², Purvi Patel³, Ajesh Desai⁴, Neeraj Jain¹

Affiliations

¹ P D Patel Institute of Applied Sciences, Charotar University of Science and Technology (CHARUSAT), Changa, India.
² Department of Obstetrics and Gynaecology, Pramukh Swami Medical College, Shree Krishna Hospital, Karamsad, India.
³ Department of Obstetrics and Gynaecology, Sir Sayajirao General Hospital and Medical College Baroda, Vadodara, India.
⁴ Department of Obstetrics and Gynaecology, GMERS Medical College and Hospital, Ahmedabad, India.

Abstract

Background: Toll-like receptor 9 (TLR9) plays a key role in the elimination of viral pathogens by recognising their CpG DNA. Polymorphisms in the TLR9 gene may influence their recognition and subsequent elimination. Therefore, the present study was designed to elucidate the role of a rare unexplored TLR9 gene polymorphism C296T/ Pro99Leu (rs5743844) in cervical cancer susceptibility among Indian women. Methods: The genotyping of TLR9 Pro99Leu polymorphism in 110 cervical cancer patients and 141 healthy controls was performed by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). Results: The genotype frequency detected in both cervical cancer and control populations was 1.0 (CC), 0.0 (CT) and 0.0 (TT); while the allele frequency was found to be 1.0 (C) and 0.0 (T). Conclusions: The present study demonstrates no involvement of TLR9 C296T/ Pro99Leu polymorphism in cervical cancer susceptibility and supports minor allele frequency (MAF) (0.0002) status of the same as no nucleotide variation was detected in any of the study subjects.

Keywords: Cervical cancer; Genotypic frequency; Polymorphism; Susceptibility; TLR9.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Case-Control Studies
Female
Gene Frequency
Genetic Predisposition to Disease*
Genotype
Humans
India
Middle Aged
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Polymorphism, Single Nucleotide*
Toll-Like Receptor 9 / genetics*
Uterine Cervical Neoplasms / genetics*
White People

Substances

TLR9 protein, human
Toll-Like Receptor 9

Grants and funding

The study was funded by Charotar University of Science and Technology (CHARUSAT).