Vesicle-Based Assays to Study Membrane Interactions of Amyloid Peptides

Methods Mol Biol. 2019:1873:39-51. doi: 10.1007/978-1-4939-8820-4_3.

Abstract

The growing interest in membrane interactions of amyloidogenic peptides and proteins emanates from the realization that lipid bilayers and membranes play central roles in the toxicity and pathological pathways of amyloid diseases. This chapter presents experimental schemes designed to study membrane interactions and membrane-induced fibrillation of amyloid peptides.

Keywords: Amyloid peptides; Amyloid-membrane interactions; Fibrillation; Lipid bilayer fluidity; Membrane dynamics; Membranes.

Publication types

  • Review

MeSH terms

  • Amyloid beta-Peptides / chemistry*
  • Amyloid beta-Peptides / metabolism
  • Biological Assay*
  • Calorimetry, Differential Scanning / methods
  • Cholesterol / chemistry
  • Cholesterol / metabolism
  • Electron Spin Resonance Spectroscopy / methods
  • Fluorescence Resonance Energy Transfer / methods
  • Fluorescent Dyes / chemistry
  • Humans
  • Islet Amyloid Polypeptide / chemistry*
  • Islet Amyloid Polypeptide / metabolism
  • Lipid Bilayers / chemistry*
  • Lipid Bilayers / metabolism
  • Membrane Fluidity
  • Peptide Fragments / chemistry*
  • Peptide Fragments / metabolism
  • Phosphatidylcholines / chemistry
  • Phosphatidylcholines / metabolism
  • Phosphatidylglycerols / chemistry
  • Phosphatidylglycerols / metabolism
  • Phosphatidylserines / chemistry
  • Phosphatidylserines / metabolism
  • Prion Proteins / chemistry*
  • Prion Proteins / metabolism
  • Spectrometry, Fluorescence / methods
  • Unilamellar Liposomes / chemistry*
  • Unilamellar Liposomes / metabolism

Substances

  • Amyloid beta-Peptides
  • Fluorescent Dyes
  • Islet Amyloid Polypeptide
  • Lipid Bilayers
  • Peptide Fragments
  • Phosphatidylcholines
  • Phosphatidylglycerols
  • Phosphatidylserines
  • Prion Proteins
  • Unilamellar Liposomes
  • amyloid beta-protein (1-42)
  • 1,2-dioleoyl-sn-glycero-3-phosphoglycerol
  • 1,2-dioleoylphosphatidylserine
  • Cholesterol
  • 1,2-oleoylphosphatidylcholine