Two novel temperate bacteriophages infecting Streptococcus pyogenes: Their genomes, morphology and stability

PLoS One. 2018 Oct 19;13(10):e0205995. doi: 10.1371/journal.pone.0205995. eCollection 2018.

Abstract

Only 3% of phage genomes in NCBI nucleotide database represent phages that are active against Streptococcus sp. With the aim to increase general awareness of phage diversity, we isolated two bacteriophages, Str01 and Str03, active against health-threatening Group A Streptococcus (GAS). Both phages are members of the Siphoviridae, but their analysis revealed that Str01 and Str03 do not belong to any known genus. We identified their structural proteins based on LC-ESI29 MS/MS and list their basic thermal stability and physico-chemical features including optimum pH. Annotated genomic sequences of the phages are deposited in GenBank (NCBI accession numbers KY349816 and KY363359, respectively).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteriophages / genetics*
  • Bacteriophages / isolation & purification
  • Bacteriophages / ultrastructure
  • Genes, Viral
  • Genome, Viral*
  • Hydrogen-Ion Concentration
  • Mass Spectrometry
  • Phylogeny
  • Streptococcus pyogenes / virology*
  • Temperature
  • Viral Proteins / metabolism
  • Virion / genetics

Substances

  • Viral Proteins

Grants and funding

This work was supported by the National Science Centre in Poland grant no. UMO-2015/18/M/NZ6/00412.