Fertility of lactating dairy cows inseminated with sex-sorted or conventional semen after Ovsynch, Presynch-Ovsynch and Double-Ovsynch protocols

Reprod Domest Anim. 2019 Feb;54(2):309-316. doi: 10.1111/rda.13363. Epub 2018 Nov 13.

Abstract

The objective was to compare pregnancy per artificial insemination (P/AI) with conventional (CS) or sex-sorted semen (SS) in dairy cows subjected to one of the three timed AI protocols. Cows (n = 356) were randomly assigned to synchronization with Ovsynch (OVS), Presynch-Ovsynch (PO) or Double-Ovsynch (DO) and inseminated on Day 77 ± 3 postpartum with either frozen-thawed SS (n = 182) or CS (n = 184) of the same bull. More cows were cyclic at the beginning of breeding Ovsynch increased (p < 0.01) with presynchronization and it was greater for DO than PO (OVS = 78.5%, PO = 85.1%, DO = 95.6%). Overall, P/AI for SS and CS increased with presynchronization (p < 0.05) on Days 31 (OVS = 35.5%, PO = 47.1%, DO = 48.3%) and 62 (OVS = 30.1%, PO = 43.8%, DO = 43.9%). Regardless of synchronization treatments, insemination with SS reduced P/AI (p < 0.02) on Days 31 (38.1% vs. 50.6%) and 62 (34.5% vs. 45.6%) compared with CS. No interaction was observed between synchronization treatment and type of semen for P/AI, although in cows receiving CS, P/AI was numerically greatest for PO (OVS = 42.0%, PO = 59.3%, DO = 49.0%), and in cows receiving SS, it was numerically greatest for those inseminated following DO (OVS = 27.9%, PO = 35.5%, DO = 47.6%). Thus, presynchronization improved P/AI in cows inseminated with sex-sorted or conventional semen.

Keywords: dairy cow; ovsynch; presynchronization; sex-sorted semen.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Cattle
  • Dinoprost / pharmacology
  • Estrus Synchronization / methods*
  • Female
  • Fertility*
  • Flow Cytometry
  • Gonadotropin-Releasing Hormone / pharmacology
  • Insemination, Artificial / veterinary*
  • Lactation*
  • Pregnancy
  • Pregnancy Rate
  • Progesterone / pharmacology
  • Random Allocation
  • Semen / drug effects*
  • Ultrasonography

Substances

  • Gonadotropin-Releasing Hormone
  • Progesterone
  • Dinoprost