Molecular systematic study of Cardamine glechomifolia Levl. (Brassicaceae) using internal transcribed spacer sequence of nuclear ribosomal DNA (ITS) and chloroplast trn L and trn L-F sequences

M Ajmal Ali; Dung Luong Van; Soo-Yong Kim

doi:10.1016/j.sjbs.2010.06.001

Molecular systematic study of Cardamine glechomifolia Levl. (Brassicaceae) using internal transcribed spacer sequence of nuclear ribosomal DNA (ITS) and chloroplast trn L and trn L-F sequences

Saudi J Biol Sci. 2010 Oct;17(4):275-290. doi: 10.1016/j.sjbs.2010.06.001. Epub 2010 Jun 15.

Authors

M Ajmal Ali¹, Dung Luong Van², Soo-Yong Kim²

Affiliations

¹ Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
² Department of Biology, University of Dalat, Dalat, Viet Nam.

Abstract

The internal transcribed spacer (ITS) region of nuclear ribosomal DNA, trnL and trnL-F genes of Cardamine glechomifolia Levl. (family Brassicaceae) were sequenced and analyzed with the sequence of related Cardamine species retrieved from NCBI GenBank to detect pattern of evolutionary differentiation. All trees resulting from combined sequence analyses data of ITS, trnL and trnL-F gene resolve that C. glechomifolia - an endemic species to South Korea clade with Cardamine microzyga (100% bootstrap support). The evolutionary history was inferred using the Maximum Parsimony method. The consistency index is (0.588235), the retention index is (0.687500), and the composite index is 0.519622 (0.404412) for all sites and parsimony-informative sites (in parentheses). The result of the analysis using Maximum Parsimony was found congruence with Maximum Likelihood method and in Baseyan analysis.

Keywords: Cardamine glechomifolia; ITS; Molecular systematic; trnL; trnL-F.