Identification of key genes in Ankylosing spondylitis

Zhong-Yang Xu; Chao Zhou; Ke-Feng Zhang; Yan-Ping Zheng

doi:10.1016/j.imlet.2018.03.016

Identification of key genes in Ankylosing spondylitis

Immunol Lett. 2018 Dec:204:60-66. doi: 10.1016/j.imlet.2018.03.016. Epub 2018 Oct 12.

Authors

Zhong-Yang Xu¹, Chao Zhou², Ke-Feng Zhang³, Yan-Ping Zheng⁴

Affiliations

¹ Department of Orthopaedics, Jining NO.1 People's Hospital, China; Department of Orthopaedics, Sandong University Qilu Hospital, China.
² Department of Orthopaedics, Sandong University Qilu Hospital, China.
³ Department of Orthopaedics, Jining NO.1 People's Hospital, China.
⁴ Department of Orthopaedics, Sandong University Qilu Hospital, China. Electronic address: zhengyanping02@163.com.

PMID: 30321561
DOI: 10.1016/j.imlet.2018.03.016

Abstract

Ankylosing spondylitis (AS) is a common form of seronegative spondyloarthritis. We had identified differentially expressed genes (DEGs) in AS based on our previous RNA-sequencing results. Our study aimed to identify key genes in AS by integrated microarray analysis. In this present study, we identified 1328 DEGs between AS and normal control by using integrated analysis of two datasets derived from the Gene Expression Omnibus (GEO) database. Functional annotation of DEGs were performed. Pathways in cancer, Pancreatic cancer and Natural killer cell mediated cytotoxicity were significantly enriched pathways for DEGs. Based on the shared DEGs of AS in both integrated analysis and our previous RNA-sequencing results, we constructed the protein and protein interaction (PPI) network. BIRC2, MAPILC3A and MAGED1 were hub proteins. Validation of gene expression by qRT-PCR were performed and the results were consistent with our integrated analysis generally. Our finding provided new clues for understanding the mechanism of AS.

Keywords: Ankylosing spondylitis (AS); Differently expressed genes; Integrated analysis; Protein-protein interaction networks.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

CD4-Positive T-Lymphocytes / immunology
CD4-Positive T-Lymphocytes / metabolism
CD8-Positive T-Lymphocytes / immunology
CD8-Positive T-Lymphocytes / metabolism
Case-Control Studies
Gene Expression Profiling
Genetic Association Studies* / methods
Genetic Predisposition to Disease*
Humans
Molecular Sequence Annotation
Protein Interaction Mapping
Protein Interaction Maps
ROC Curve
Reproducibility of Results
Spondylitis, Ankylosing / genetics*
Spondylitis, Ankylosing / immunology
Spondylitis, Ankylosing / metabolism
Transcriptome
Tumor Necrosis Factor-alpha / metabolism

Substances

Tumor Necrosis Factor-alpha