Response surface optimization of lipid and protein extractions from Spirulina platensis using ultrasound assisted osmotic shock method

H Hadiyanto; Nais Pinta Adetya

doi:10.1007/s10068-018-0389-y

Response surface optimization of lipid and protein extractions from Spirulina platensis using ultrasound assisted osmotic shock method

Food Sci Biotechnol. 2018 May 29;27(5):1361-1368. doi: 10.1007/s10068-018-0389-y. eCollection 2018 Oct.

Authors

H Hadiyanto^{1

2}, Nais Pinta Adetya¹

Affiliations

¹ 1Department of Chemical Engineering, Faculty of Engineering, Diponegoro University, Jl. Prof Soedarto, SH Tembalang, Semarang, 50275 Indonesia.
² 2Master Program of Environmental Science, School of Postgraduate Studies, Diponegoro University, Jl. Imam Bardjo No 3-5, Semarang, Indonesia.

Abstract

In this study, we optimized the process for extracting lipids and proteins from wet biomasses of Spirulina sp. using a 4-kHz ultrasonic osmotic shock method with ultrasound enhancement at a constant frequency of 40 kHz. Optimization was conducted using a response surface methodology (RSM) at an osmotic NaCl concentration of 10-30%, solvent:biomass ratio of 5-15 v/w, and extraction times of 20-50 min. The present osmotic shock method with ultrasound irradiation increased lipid yields to 6.65% in the presence of 11.9% NaCl, a solvent:biomass ratio of 12:1 v/w, and a 22-min extraction time, and protein yields to 43.96% with 15.12% NaCl, a solvent:biomass ratio of 10:1 v/w, and a 30-min extraction time.

Keywords: Extraction; Lipid; Microalgae; Osmotic shock; Ultrasound.