X-Ray Absorption Spectroscopy of Metalloproteins

Limei Zhang

doi:10.1007/978-1-4939-8864-8_12

X-Ray Absorption Spectroscopy of Metalloproteins

Methods Mol Biol. 2019:1876:179-195. doi: 10.1007/978-1-4939-8864-8_12.

Author

Limei Zhang^{1

2

3}

Affiliations

¹ Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, NE, USA. limei.zhang@unl.edu.
² Redox Biology Center, University of Nebraska-Lincoln, Lincoln, NE, USA. limei.zhang@unl.edu.
³ Nebraska Center for Integrated Biomolecular Communication, University of Nebraska-Lincoln, Lincoln, NE, USA. limei.zhang@unl.edu.

PMID: 30317482
DOI: 10.1007/978-1-4939-8864-8_12

Abstract

X-ray absorption spectroscopy (XAS) has been widely used as a powerful local structural tool for studying the metal binding sites in metalloproteins, owing to its element specificity, chemical sensitivity, and minimum requirement for sample preparation. The principle subject of this chapter is to provide a general introduction of this technique to molecular biologists and biochemists, with the intention to fill the knowledge gaps in interdisciplinary communication and collaboration. An update of the XAS-based technique applied recently to metalloproteins is also briefly introduced at the end of the chapter.

Keywords: Extended X-ray absorption fine structure (EXAFS); Metalloclusters; Metalloproteins; X-ray absorption near edge spectrum (XANES); X-ray absorption spectroscopy (XAS).

MeSH terms

Binding Sites
Metalloproteins / chemistry*
Metalloproteins / metabolism
Spectrometry, X-Ray Emission
X-Ray Absorption Spectroscopy / methods*

Substances

Metalloproteins