Efficient mRNA delivery system utilizing chimeric VSVG-L7Ae virus-like particles

Biochem Biophys Res Commun. 2018 Nov 10;505(4):1097-1102. doi: 10.1016/j.bbrc.2018.09.113. Epub 2018 Oct 10.

Abstract

The delivery of mRNA is advantageous over DNA delivery as it is transient and does not carry the risk of genomic DNA integration. However, there are currently few efficient mRNA delivery options available, especially for hard-to-transfect cell types, and thus new delivery methods are needed. To this end, we have established a novel mRNA delivery system utilizing chimeric virus-like particles (VLPs). We generated a novel VLP by fusing protein G of Vesicular stomatitis virus (VSV-G) with a ribosomal protein L7Ae of Archeoglobus fulgidus. This system allowed the efficient delivery of EGFP mRNA which was independent from the presence of BoxC/D motif in the mRNA sequence. Our VSVG-L7Ae VLP system demonstrated high transduction efficacy in hard-to-transfect cell lines, such as human induced pluripotent stem cells (iPS cells) and monocytes. In summary, this platform may serve as an efficient and transient transgene delivery tool for an mRNA of interest.

Keywords: L7Ae RNA-binding protein; VLPs; mRNA delivery.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Gene Transfer Techniques*
  • HEK293 Cells
  • Humans
  • Membrane Glycoproteins / chemistry*
  • RNA, Messenger / genetics*
  • Ribosomal Proteins / chemistry*
  • Viral Envelope Proteins / chemistry*

Substances

  • G protein, vesicular stomatitis virus
  • Membrane Glycoproteins
  • RNA, Messenger
  • Ribosomal Proteins
  • Viral Envelope Proteins