Overview of the Maturation Machinery of the H-Cluster of [FeFe]-Hydrogenases with a Focus on HydF

Int J Mol Sci. 2018 Oct 11;19(10):3118. doi: 10.3390/ijms19103118.

Abstract

Hydrogen production in nature is performed by hydrogenases. Among them, [FeFe]-hydrogenases have a peculiar active site, named H-cluster, that is made of two parts, synthesized in different pathways. The cubane sub-cluster requires the normal iron-sulfur cluster maturation machinery. The [2Fe] sub-cluster instead requires a dedicated set of maturase proteins, HydE, HydF, and HydG that work to assemble the cluster and deliver it to the apo-hydrogenase. In particular, the delivery is performed by HydF. In this review, we will perform an overview of the latest knowledge on the maturation machinery of the H-cluster, focusing in particular on HydF.

Keywords: HydE; HydF; HydG; [FeFe]-hydrogenases; hydrogenases; maturases.

Publication types

  • Review

MeSH terms

  • Catalysis
  • Hydrogen / chemistry*
  • Hydrogen / metabolism*
  • Hydrogenase / chemistry*
  • Hydrogenase / classification
  • Hydrogenase / metabolism*
  • Iron / chemistry*
  • Iron / metabolism*
  • Models, Molecular
  • Protein Binding
  • Protein Interaction Domains and Motifs
  • Structure-Activity Relationship

Substances

  • Hydrogen
  • Iron
  • Hydrogenase