Aminobenzosuberone Scaffold as a Modular Chemical Tool for the Inhibition of Therapeutically Relevant M1 Aminopeptidases

Molecules. 2018 Oct 11;23(10):2607. doi: 10.3390/molecules23102607.

Abstract

The synthesis of racemic substituted 7-amino-5,7,8,9-tetrahydrobenzocyclohepten-6-one hydrochlorides was optimized to enhance reproducibility and increase the overall yield. In order to investigate their specificity, series of enzyme inhibition assays were carried out against a diversity of proteases, covering representative members of aspartic, cysteine, metallo and serine endopeptidases and including eight members of the monometallic M1 family of aminopeptidases as well as two members of the bimetallic M17 and M28 aminopeptidase families. This aminobenzosuberone scaffold indeed demonstrated selective inhibition of M1 aminopeptidases to the exclusion of other tested protease families; it was particularly potent against mammalian APN and its bacterial/parasitic orthologues EcPepN and PfAM1.

Keywords: M1 aminopeptidases; aminobenzosuberone scaffold; selective inhibitors.

MeSH terms

  • Aminopeptidases / antagonists & inhibitors*
  • Aminopeptidases / chemistry*
  • Animals
  • Coumarins / chemistry*
  • Coumarins / pharmacology*
  • Enzyme Activation / drug effects
  • Molecular Conformation
  • Molecular Docking Simulation
  • Molecular Dynamics Simulation
  • Molecular Structure
  • Protease Inhibitors / chemistry*
  • Protease Inhibitors / pharmacology*
  • Protein Binding
  • Structure-Activity Relationship
  • Substrate Specificity

Substances

  • Coumarins
  • Protease Inhibitors
  • benzosuberone
  • Aminopeptidases