β-Agarase immobilized on tannic acid-modified Fe3O4 nanoparticles for efficient preparation of bioactive neoagaro-oligosaccharide

Qiong Xiao; Chunli Liu; Hui Ni; Yanbing Zhu; Zedong Jiang; Anfeng Xiao

doi:10.1016/j.foodchem.2018.08.017

β-Agarase immobilized on tannic acid-modified Fe₃O₄ nanoparticles for efficient preparation of bioactive neoagaro-oligosaccharide

Food Chem. 2019 Jan 30:272:586-595. doi: 10.1016/j.foodchem.2018.08.017. Epub 2018 Aug 4.

Authors

Qiong Xiao¹, Chunli Liu¹, Hui Ni¹, Yanbing Zhu¹, Zedong Jiang¹, Anfeng Xiao²

Affiliations

¹ College of Food and Biological Engineering, Jimei University, Xiamen 361021, China; Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Xiamen 361021, China; Fujian Provincial Engineering Technology Research Center of Marine Functional Food, Xiamen, Fujian Province 361021, China; Xiamen Key Laboratory of Marine Functional Food, Xiamen 361021, China.
² College of Food and Biological Engineering, Jimei University, Xiamen 361021, China; Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Xiamen 361021, China; Fujian Provincial Engineering Technology Research Center of Marine Functional Food, Xiamen, Fujian Province 361021, China; Xiamen Key Laboratory of Marine Functional Food, Xiamen 361021, China. Electronic address: xxaaffeng@jmu.edu.cn.

PMID: 30309586
DOI: 10.1016/j.foodchem.2018.08.017

Abstract

β-Agarase was immobilized by using tannic acid modified-Fe₃O₄ magnetic nanoparticles (TA-MNPs) as a supportmaterial. The MNPs were synthesized by improved chemical coprecipitation method and modified with TA for agarase immobilization. TA-MNPs and immobilized β-agarase were characterized by transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and thermal gravimetric analysis (TGA), all of which indicated the successful surface modification of MNPs with TA and the immobilization of β-agarase. The optimal immobilization conditions for 25 mg TA-MNPs included 100 r/min oscillation speed, immobilization time of 2 h, immobilization temperature of 15 °C, and initial β-agarase concentration of 3 mL (480 U). Immobilized β-agarase showed better pH and thermal stability and excellent reusability than the free enzyme. Results revealed the promising application of β-agarase-TA-MNPs for the preparation of neoagaro-oligosaccharides with different averagepolymerizationdegrees and varying activities in the antioxidant.

Keywords: Characterization; Immobilization; Neoagaro-oligosaccharides; Tannic acid-modified Fe(3)O(4) nanoparticles; β-Agarase.

MeSH terms

Antioxidants / chemistry
Enzyme Stability
Enzymes, Immobilized / chemistry
Enzymes, Immobilized / metabolism
Ferrosoferric Oxide / chemistry
Glycoside Hydrolases / chemistry
Glycoside Hydrolases / metabolism*
Hydrogen-Ion Concentration
Kinetics
Magnetite Nanoparticles / chemistry*
Microscopy, Electron, Transmission
Oligosaccharides / chemical synthesis
Oligosaccharides / chemistry*
Spectroscopy, Fourier Transform Infrared
Tannins / chemistry*
Temperature
Thermogravimetry

Substances

Antioxidants
Enzymes, Immobilized
Magnetite Nanoparticles
Oligosaccharides
Tannins
Glycoside Hydrolases
agarase
Ferrosoferric Oxide