MiR-214 inhibits snakehead vesiculovirus (SHVV) replication by targeting host GS

Chi Zhang; Ningqiu Li; Xiaozhe Fu; Qiang Lin; Li Lin; Jiagang Tu

doi:10.1016/j.fsi.2018.10.028

MiR-214 inhibits snakehead vesiculovirus (SHVV) replication by targeting host GS

Fish Shellfish Immunol. 2019 Jan:84:299-303. doi: 10.1016/j.fsi.2018.10.028. Epub 2018 Oct 9.

Authors

Chi Zhang¹, Ningqiu Li², Xiaozhe Fu², Qiang Lin², Li Lin³, Jiagang Tu⁴

Affiliations

¹ Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Fishery Drug Development, Ministry of Agriculture, Key Laboratory of Aquatic Animal Immune Technology, Guangdong Provinces, Guangzhou, 510380, China; Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.
² Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Fishery Drug Development, Ministry of Agriculture, Key Laboratory of Aquatic Animal Immune Technology, Guangdong Provinces, Guangzhou, 510380, China.
³ Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei, 430070, China; Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, Guangdong Provincial Key Laboratory of Waterfowl Healthy Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, China. Electronic address: linli@zhku.edu.cn.
⁴ Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Fishery Drug Development, Ministry of Agriculture, Key Laboratory of Aquatic Animal Immune Technology, Guangdong Provinces, Guangzhou, 510380, China; Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei, 430070, China; Hubei Engineering Technology Research Center for Aquatic Animal Diseases Control and Prevention, Wuhan, Hubei, 430070, China. Electronic address: tujiagang@mail.hzau.edu.cn.

PMID: 30308292
DOI: 10.1016/j.fsi.2018.10.028

Abstract

MicroRNAs (miRNAs) are small noncoding RNAs that have been reported to play important roles in virus replication. Snakehead vesiculovirus (SHVV) is a new rhabdovirus isolated from diseased hybrid snakehead and has caused heavy economical losses in cultured snakehead fish in China. Our previous study has revealed that miR-214 inhibited SHVV replication, but the underline mechanism was not completely understood. In this study, glycogen synthase (GS) gene was identified as a target gene of miR-214. Overexpression of miR-214 reduced cellular GS gene expression. Knockdown of GS by siRNA, similar to the overexpression of miR-214, inhibited SHVV replication. Moreover, we found that siGS-mediated inhibition of SHVV replication could be restored by reducing cellular miR-214 level via using miR-214 inhibitor, indicating that miR-214 inhibited SHVV replication at least partially via targeting GS. This study provided information for understanding the molecular mechanism of SHVV pathogenicity and a potential antiviral strategy against SHVV infection.

Keywords: Glycogen synthase; Nakehead vesiculovirus; Replication; miR-214; microRNA.

MeSH terms

Animals
Fish Diseases / physiopathology*
Fish Diseases / virology
Fish Proteins / genetics*
Fish Proteins / metabolism
Glycogen Synthase / genetics*
Glycogen Synthase / metabolism
MicroRNAs / genetics*
MicroRNAs / metabolism
Perciformes*
RNA, Viral / genetics*
RNA, Viral / metabolism
Rhabdoviridae Infections / physiopathology
Rhabdoviridae Infections / veterinary*
Rhabdoviridae Infections / virology
Vesiculovirus / genetics
Vesiculovirus / physiology

Substances

Fish Proteins
MicroRNAs
RNA, Viral
Glycogen Synthase