Expression Analysis of Long Non-Coding RNA HAR1A and HAR1B in HBV-Induced Hepatocullular Carcinoma in Chinese Patients

Zhenjing Shi; Ya Luo; Minghui Zhu; Yu Zhou; Bingru Zheng; Daoyi Wu; Shuting Wang; Xiangbang Xie; Heping Lin; Xixiang Yu

doi:10.1093/labmed/lmy055

Expression Analysis of Long Non-Coding RNA HAR1A and HAR1B in HBV-Induced Hepatocullular Carcinoma in Chinese Patients

Lab Med. 2019 Apr 8;50(2):150-157. doi: 10.1093/labmed/lmy055.

Authors

Zhenjing Shi¹, Ya Luo¹, Minghui Zhu², Yu Zhou³, Bingru Zheng¹, Daoyi Wu⁴, Shuting Wang⁵, Xiangbang Xie¹, Heping Lin⁶, Xixiang Yu⁷

Affiliations

¹ Department of Intervention, Third Affiliated Hospital of Wenzhou Medical University, Ruian, China.
² Department of Hepatobiliary Surgery, Wenzhou People's Hospital, China.
³ Department of Infectious Diseases, Third Affiliated Hospital of Wenzhou Medical University, Ruian, China.
⁴ Department of Gastrointestinal Surgery, Third Affiliated Hospital of Wenzhou Medical University, Ruian, China.
⁵ Department of Imaging and Intervention, Zhejiang Chinese Medicine Hospital, Hangzhou, China.
⁶ Department of Respiratory Diseases, Third Affiliated Hospital of Wenzhou Medical University, Ruian, China.
⁷ Department of Vasointerventional Surgery, Wenzhou People's Hospital, China.

PMID: 30304523
DOI: 10.1093/labmed/lmy055

Abstract

Objective: To determine the clinical relevance of long noncoding RNA (lncRNA) HAR1A and HAR1B expression in hepatocellular carcinoma (HCC).

Methods: In this study, we enrolled 50 cases of chronic hepatitis B (CHB) without cirrhosis, 50 cases of CHB and liver cirrhosis (LC), and 100 cases of HBV and HCC. The expression profiles of lncRNA HAR1A and HAR1B were analyzed using reverse transcription-quantitative polymerase chain reaction (RT-qPCR).

Results: The expression levels of HAR1A and HAR1B were significantly lower in the HCC group, compared with the CHB and LC groups (P <.01). HAR1A and HAR1B were negatively associated with histologic grade and TNM (tumor/nodes/metastasis) stage (all P <.05). Univariable multivariable analysis showed that decreased HAR1A (HR = 0.753, P = .02) and HAR1B (HR = 0.551, P = .01) levels were independent predictors for shorter overall survival (OS) in HCC.

Conclusion: Decreased HAR1A and HAR1B expression in HCC indicates poor prognosis.

Keywords: HAR1A; HAR1B; RT-qPCR; expression; hepatocellular carcinoma; survival.

MeSH terms

Aged
Cohort Studies
Female
Gene Expression Profiling
Gene Expression Regulation, Neoplastic / genetics
Hepatitis B, Chronic / complications
Hepatitis B, Chronic / epidemiology
Hepatitis B, Chronic / genetics*
Hepatitis B, Chronic / metabolism
Humans
Liver Cirrhosis / complications
Liver Cirrhosis / epidemiology
Liver Cirrhosis / genetics*
Liver Cirrhosis / metabolism
Liver Neoplasms / complications
Liver Neoplasms / epidemiology
Liver Neoplasms / genetics*
Liver Neoplasms / metabolism
Male
Middle Aged
RNA, Untranslated / analysis
RNA, Untranslated / genetics*
RNA, Untranslated / metabolism
Real-Time Polymerase Chain Reaction
Transcriptome / genetics

Substances

HAR1 noncoding RNA, human
RNA, Untranslated