Abstract
The formation of a complex between Zn(II) and beta-D-fructose 2,6-bisphosphate was shown because the latter compound: activated bis(5'-guanosyl)tetraphosphatase (EC 3.6.1.17) and dinucleoside triphosphatase (EC 3.6.1.29) only to the extent that they could be inhibited by Zn(II); increased the consumption of Zn(II) necessary to titrate to an end point a solution of the metallochromic indicator eriochrome black T; coeluted with Zn(II) in a gel filtration column capable of resolving them if unbound. Neither of those effects was shown by D-fructose 1,6-bisphosphate under the same conditions.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acid Anhydride Hydrolases*
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Animals
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Enzyme Activation
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Fructosediphosphates* / pharmacology*
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Hexosediphosphates* / pharmacology*
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Kinetics
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Liver / enzymology
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Organometallic Compounds / pharmacology*
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Phosphoric Monoester Hydrolases / isolation & purification
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Phosphoric Monoester Hydrolases / metabolism*
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Rats
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Zinc* / pharmacology
Substances
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Fructosediphosphates
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Hexosediphosphates
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Organometallic Compounds
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fructose 2,6-bisphosphate-zinc(II) complex
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fructose 2,6-diphosphate
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Phosphoric Monoester Hydrolases
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Acid Anhydride Hydrolases
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bis(5'-nucleosyl)tetraphosphatase (asymmetrical)
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bis(5'-adenosyl)triphosphatase
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Zinc