Chemical Constituents from the Stems of Tinospora sinensis and Their Bioactivity

Sio-Hong Lam; Po-Hsun Chen; Hsin-Yi Hung; Tsong-Long Hwang; Chih-Chao Chiang; Tran Dinh Thang; Ping-Chung Kuo; Tian-Shung Wu

doi:10.3390/molecules23102541

Chemical Constituents from the Stems of Tinospora sinensis and Their Bioactivity

Molecules. 2018 Oct 5;23(10):2541. doi: 10.3390/molecules23102541.

Authors

Sio-Hong Lam¹, Po-Hsun Chen², Hsin-Yi Hung³, Tsong-Long Hwang^{4

5

6}, Chih-Chao Chiang^{7

8

9}, Tran Dinh Thang^{10

11}, Ping-Chung Kuo¹², Tian-Shung Wu^{13

14}

Affiliations

¹ School of Pharmacy, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan. shlam@mail.ncku.edu.tw.
² Department of Biotechnology, National Formosa University, Yunlin 632, Taiwan. pcckuoo@gmail.com.
³ School of Pharmacy, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan. z10308005@email.ncku.edu.tw.
⁴ Graduate Institute of Natural Products, School of Traditional Chinese Medicine, College of Medicine, Chang Gung University, Taoyuan 333, Taiwan. htl@mail.cgu.edu.tw.
⁵ Research Center for Chinese Herbal Medicine, Research Center for Food and Cosmetic Safety, Graduate Institute of Health Industry Technology, College of Human Ecology, Chang Gung University of Science and Technology, Taoyuan 333, Taiwan. htl@mail.cgu.edu.tw.
⁶ Department of Anesthesiology, Chang Gung Memorial Hospital, Taoyuan 333, Taiwan. htl@mail.cgu.edu.tw.
⁷ Graduate Institute of Clinical Medical Sciences, College of Medicine, Chang Gung University, Taoyuan 338, Taiwan. moonlight0604@hotmail.com.
⁸ Supervisor board, Taoyuan Chinese Medicine Association, Taoyuan 338, Taiwan. moonlight0604@hotmail.com.
⁹ Dazhu Fengze Chinese Medicine Clinic, Taoyuan 338, Taiwan. moonlight0604@hotmail.com.
¹⁰ School of Chemistry, Biology and Environment, Vinh University, Vinh City 44000, Vietnam. thangtd@vinhuni.edu.vn.
¹¹ NTT Institute of High Technology, Nguyen Tat Thanh University, Ho Chi Minh City 700000, Vietnam. thangtd@vinhuni.edu.vn.
¹² School of Pharmacy, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan. z10502016@email.ncku.edu.tw.
¹³ School of Pharmacy, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan. tswu@mail.ncku.edu.tw.
¹⁴ Department of Pharmacy, College of Pharmacy and Health Care, Tajen University, Pingtung 907, Taiwan. tswu@mail.ncku.edu.tw.

Abstract

Fifty-seven compounds were purified from the stems of Tinospora sinensis, including three new compounds characterized as a lignan (1), a pyrrole alkaloid (11), and a benzenoid (17), respectively. Their structures were elucidated and established by various spectroscopic and spectrometric analytical methods. Among the isolates, fifteen compounds were examined for their anti-inflammatory potential in vitro. The results showed that several compounds displayed moderate inhibition of N-formyl-methionyl-leucyl-phenylalanine/cytochalasin B (fMLP/CB)-induced superoxide anion generation and elastase release.

Keywords: Menispermaceae; elastase release.; lignan; pyrrole alkaloid; superoxide anion generation.

MeSH terms

Alkaloids / chemistry
Alkaloids / pharmacology*
Cytochalasin B / antagonists & inhibitors
Cytochalasin B / toxicity
Humans
Lignans / chemistry
Lignans / pharmacology*
Molecular Structure
Neutrophils / drug effects
Neutrophils / enzymology
Pancreatic Elastase / biosynthesis
Pancreatic Elastase / drug effects
Pancreatic Elastase / metabolism*
Plant Stems / chemistry
Pyrroles / chemistry
Pyrroles / pharmacology*
Superoxides / antagonists & inhibitors
Superoxides / toxicity
Tinospora / chemistry

Substances

Alkaloids
Lignans
Pyrroles
Superoxides
Cytochalasin B
Pancreatic Elastase

Grants and funding

MOST 106 - 2320 - B - 006 - 073; CMRPF1G0241~3, CMRPF1F0061~3, and BMRP450/Ministry of Science and Technology (MOST), Chang Gung Memorial Hospital