The Expanding Molecular Genetics Tool Kit in Chlamydia

Raphael H Valdivia; Robert J Bastidas

doi:10.1128/JB.00590-18

The Expanding Molecular Genetics Tool Kit in Chlamydia

J Bacteriol. 2018 Nov 26;200(24):e00590-18. doi: 10.1128/JB.00590-18. Print 2018 Dec 15.

Authors

Raphael H Valdivia¹, Robert J Bastidas²

Affiliations

¹ Department of Molecular Genetics and Microbiology and Center for Host-Microbial Interactions, Duke University School of Medicine, Durham, North Carolina, USA.
² Department of Molecular Genetics and Microbiology and Center for Host-Microbial Interactions, Duke University School of Medicine, Durham, North Carolina, USA robert.bastidas@duke.edu.

Abstract

Chlamydia has emerged as an important model system for the study of host pathogen interactions, in part due to a resurgence in the development of tools for its molecular genetic manipulation. An additional tool, published by Keb et al. (G. Keb, R. Hayman, and K. A. Fields, J. Bacteriol. 200:e00479-18, 2018, https://doi.org/10.1128/JB.00479-18), now allows for custom genetic engineering of genomic regions that were traditionally recalcitrant to genetic manipulation, such as genes within operons. This new method will be an essential instrument for the elucidation of Chlamydia-host interactions.

Keywords: Chlamydia genetics; Chlamydia trachomatis.

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MeSH terms

Alleles
Chlamydia Infections*
Chlamydia trachomatis*
Gene Deletion
Host-Pathogen Interactions
Humans