Objective: To investigate the changes of E-selectin, thrombin-antithrombin complex(TAT), prothrombin fragment 1+2(F1+2), tissue factor(TF)and tissue factor pathway inhibitor(TFPI)before and one year after splenectomy in thalassemia patients.
Methods: A total of 30 thalassemia patients undergoing electric laparoscopic splenectomy and 30 normal controls(NC) were enrolled in the study.Plasma levels of E-selectin, TAT, F1+2, TF and TFPI were detected by enzyme-linked immuno sorbent assay(ELISA).
Results: One year after splenectomy,the plasma concentrations of E-selectin, TAT, F1+2, TF, TFPI were significantly higher than those in both preoperative and NC groups.Levels of E-selectin, TAT, F1+2 before splenectomy were significantly higher than those in NC groups. In addition, there was a positive correlation between plasma TF and TFPI level before and after splenectomy, and the levels of TF and TFPI positively correlated with TAT and F1+2, respectively.
Conclusion: After splenectomy, the platelet count increases, the activity of endothelial cells is injured, the procoagulant factor increases, the blood is in procoagulant state, the TF/TFPI shows an importent role in the thrombosis of thalassemia patieints after splenectomy and may be used to evaluate the prothrombotic state of this diasease.