Response of Pseudomonas fluorescens to dimethyl phthalate

Zhigang Wang; Chunlong Wang; Yimin You; Weihui Xu; Zhihang Lv; Zeping Liu; Wenjing Chen; Yiran Shi; Junhe Wang

doi:10.1016/j.ecoenv.2018.09.078

Response of Pseudomonas fluorescens to dimethyl phthalate

Ecotoxicol Environ Saf. 2019 Jan 15:167:36-43. doi: 10.1016/j.ecoenv.2018.09.078. Epub 2018 Oct 4.

Authors

Zhigang Wang¹, Chunlong Wang², Yimin You³, Weihui Xu⁴, Zhihang Lv⁵, Zeping Liu⁶, Wenjing Chen⁷, Yiran Shi⁸, Junhe Wang⁹

Affiliations

¹ School of Life Science and Agriculture and Forestry, Qiqihar University, Qiqihar, Heilongjiang, 161006, China. Electronic address: zhigang_wang01@qqhru.edu.cn.
² School of Life Science and Agriculture and Forestry, Qiqihar University, Qiqihar, Heilongjiang, 161006, China. Electronic address: chunlong_wang@qqhru.edu.cn.
³ School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address: yiminy@sjtu.edu.cn.
⁴ School of Life Science and Agriculture and Forestry, Qiqihar University, Qiqihar, Heilongjiang, 161006, China. Electronic address: weihui_xu@qqhru.edu.cn.
⁵ School of Life Science and Agriculture and Forestry, Qiqihar University, Qiqihar, Heilongjiang, 161006, China. Electronic address: zhihang_lv@qqhru.edu.cn.
⁶ School of Life Science and Agriculture and Forestry, Qiqihar University, Qiqihar, Heilongjiang, 161006, China. Electronic address: zeping_liu@qqhru.edu.cn.
⁷ School of Life Science and Agriculture and Forestry, Qiqihar University, Qiqihar, Heilongjiang, 161006, China. Electronic address: wenjing_chen@qqhru.edu.cn.
⁸ School of Life Science and Agriculture and Forestry, Qiqihar University, Qiqihar, Heilongjiang, 161006, China. Electronic address: yiran_shi@qqhru.edu.cn.
⁹ Qiqihar Branch of Heilongjiang Academy of Agricultural Sciences, Qiqihar, Heilongjiang, 161006, China. Electronic address: jhwang@caas.cn.

PMID: 30292974
DOI: 10.1016/j.ecoenv.2018.09.078

Abstract

Dimethyl phthalate (DMP) is a ubiquitous pollutant that is very harmful to organisms due to its mutagenicity, teratogenicity and carcinogenicity. Pseudomonas fluorescens (P. fluorescens) is one of the most important bacteria in the environment. In this study, the response of P. fluorescens to DMP was investigated. It was found that DMP greatly inhibited the growth and glucose utilization of P. fluorescens when the concentration of DMP was ranged from 20 to 40 mg/l. The surface hydrophobicity and membrane permeability of P. fluorescens were also increased by DMP. DMP could lead to the deformations of cell membrane and the mis-opening of membrane channels. RNA-Seq and RT-qPCR results showed that the expression of some genes in P. fluorescens were altered, including the genes involved in energy metabolism, ATP-binding cassette (ABC) transporting and two-component systems. Additionally, the productions of lactic acid and pyruvic acid were reduced and the activity of hexokinase was inhibited in P. fluorescens by DMP. Clearly, the results suggested that DMP contamination could alter the biological function of P. fluorescens in the environment.

Keywords: ATP-binding cassette; Membrane damage; Phthalic acid esters; RNA-Seq; Two-component systems.

MeSH terms

ATP-Binding Cassette Transporters / genetics
Dose-Response Relationship, Drug
Gene Expression Regulation, Bacterial
Hydrophobic and Hydrophilic Interactions
Mutagens / toxicity
Phthalic Acids / toxicity*
Pseudomonas fluorescens / drug effects*
Pseudomonas fluorescens / genetics
Sequence Analysis, RNA
Transcriptome

Substances

ATP-Binding Cassette Transporters
Mutagens
Phthalic Acids
dimethyl phthalate