Doehlert design in the optimization of ultrasound assisted dissolution of fish fillet samples with tetramethyl ammonium hydroxide for metals determination using FAAS

Daniélen Dos Santos Silva; Carine Souza Dos Santos; Luzia Aparecida Pando; Mário Sérgio Rocha Gomes; Cleber Galvão Novaes; Walter Nei Lopes Dos Santos; Marcos Almeida Bezerra

doi:10.1016/j.foodchem.2018.02.049

Doehlert design in the optimization of ultrasound assisted dissolution of fish fillet samples with tetramethyl ammonium hydroxide for metals determination using FAAS

Food Chem. 2019 Feb 1:273:71-76. doi: 10.1016/j.foodchem.2018.02.049. Epub 2018 Feb 13.

Authors

Daniélen Dos Santos Silva¹, Carine Souza Dos Santos¹, Luzia Aparecida Pando¹, Mário Sérgio Rocha Gomes¹, Cleber Galvão Novaes¹, Walter Nei Lopes Dos Santos², Marcos Almeida Bezerra³

Affiliations

¹ Departamento de Ciências e Tecnologias, Universidade Estadual do Sudoeste da Bahia, Rua José Moreira Sobrinho, s/n, 45208-091 Jequié, BA, Brazil.
² Departamento de Ciências Exatas e da Terra, Universidade do Estado da Bahia, Rua Silveira Martins, 2555, 40170-290 Salvador, BA, Brazil; Departamento de Química Analítica, Universidade Federal da Bahia, Instituto de Química, Rua Barão de Jeremoabo, 147, 40170-115 Salvador, BA, Brazil.
³ Departamento de Ciências e Tecnologias, Universidade Estadual do Sudoeste da Bahia, Rua José Moreira Sobrinho, s/n, 45208-091 Jequié, BA, Brazil. Electronic address: mbezerra@uesb.edu.br.

PMID: 30292377
DOI: 10.1016/j.foodchem.2018.02.049

Abstract

Doehlert design and desirability function were used in the optimization of an ultrasound assisted dissolution method of fish fillet samples with tetramethyl ammonium hydroxide (TMAH) for the determination of Ca, Fe, Zn and Mg by flame atomic absorption spectrometry. The variables values after optimization were 620 μL (volume of TMAH), 25 min (sonication time) and 46 °C (bath temperature). The quantification limits for Ca, Mg, Fe and Zn were 56, 107, 23 and 2.9 μg g^-1, respectively. Accuracy was evaluated by comparing the results generated from the analysis of the samples treated by the proposed method and by wet digestion. Additionally, accuracy for Fe and Zn was assessed by the analysis of certified reference materials Tort-3 (Lobster hepatopancreas), ERM-CE 278 (mussel tissue) and Dolt-4 (Fish liver). There were no significant differences between the results obtained at a 95% confidence level when results were evaluated by t-test application.

Keywords: Doehlert design; Fish fillets; Metals; Overall desirability; TMAH; Ultrasound.

MeSH terms

Animals
Fish Products / analysis*
Food Analysis / methods*
Food Analysis / statistics & numerical data
Food Contamination / analysis
Metals / analysis*
Models, Statistical
Quaternary Ammonium Compounds / chemistry*
Solubility
Spectrophotometry, Atomic / methods*
Temperature
Ultrasonics / methods

Substances

Metals
Quaternary Ammonium Compounds
tetramethylammonium