Comparison of sampling methods for profiling cervicovaginal microbiome in rhesus macaques

Brian A Schmidt; Ronald Phillips; Matthew Rolston; Reben Raeman; Smita S Iyer

doi:10.1111/jmp.12381

Comparison of sampling methods for profiling cervicovaginal microbiome in rhesus macaques

J Med Primatol. 2019 Feb;48(1):54-57. doi: 10.1111/jmp.12381. Epub 2018 Oct 2.

Authors

Brian A Schmidt¹, Ronald Phillips¹, Matthew Rolston², Reben Raeman³, Smita S Iyer^{1

4

5}

Affiliations

¹ Center for Comparative Medicine, University of California, Davis, California.
² Host Microbe Systems Biology Core, University of California, Davis, California.
³ Department of Pathology, University of Pittsburgh, Pittsburgh, Pennsylvania.
⁴ California National Primate Research Center, University of California, Davis, California.
⁵ Department of Pathology, Microbiology, and Immunology, School of Veterinary Medicine, University of California, Davis, California.

Abstract

Cervicovaginal bacteria cause inflammation which in turn increases HIV risk. Profiling the cervicovaginal microbiome, therefore, is instrumental for vaccine development. We show that the microbiome profile captured by cervicovaginal lavage is comparable to samples obtained by vaginal swabs. Thus, lavage may serve as a sampling strategy in NHP vaccine studies.

Keywords: 16S rRNA; HIV; lavage; swab.

Publication types

Comparative Study
Research Support, N.I.H., Extramural

MeSH terms

Animals
Bacteria / isolation & purification*
Cervix Uteri / microbiology*
Female
Macaca mulatta / microbiology*
Microbiota*
Specimen Handling / methods
Specimen Handling / veterinary*
Vagina / microbiology*

Abstract

Publication types

MeSH terms

Grants and funding