Fractionation Techniques to Increase Plant Proteome Coverage: Combining Separation in Parallel at the Protein and the Peptide Level

Methods Mol Biol. 2019:1871:93-105. doi: 10.1007/978-1-4939-8814-3_6.

Abstract

Peptide spectral libraries enable targeted identification and quantitation of low-abundance proteins in a complex plant proteome. Here we describe parallel protein and peptide fractionation techniques to improve plant proteome coverage and facilitate construction of spectral libraries.

Keywords: C18; PEG; Peptide fractionation; Plant proteomics; Protein fractionation; SCX.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chemical Fractionation* / methods
  • Peptides / analysis
  • Peptides / isolation & purification
  • Plant Proteins / analysis
  • Plant Proteins / isolation & purification*
  • Proteome*
  • Proteomics* / methods
  • Solvents

Substances

  • Peptides
  • Plant Proteins
  • Proteome
  • Solvents