Fyn Regulates Binding Partners of Cyclic-AMP Dependent Protein Kinase A

Anna M Schmoker; Samuel A Barritt; Marion E Weir; Jacqueline E Mann; Tyler C Hogan; Bryan A Ballif; Paula B Deming

doi:10.3390/proteomes6040037

Fyn Regulates Binding Partners of Cyclic-AMP Dependent Protein Kinase A

Proteomes. 2018 Sep 29;6(4):37. doi: 10.3390/proteomes6040037.

Authors

Anna M Schmoker¹, Samuel A Barritt^{2

3}, Marion E Weir⁴, Jacqueline E Mann⁵, Tyler C Hogan⁶, Bryan A Ballif⁷, Paula B Deming⁸

Affiliations

¹ Department of Biology, University of Vermont, Burlington, VT 05405, USA. aschmoke@uvm.edu.
² Department of Biology, University of Vermont, Burlington, VT 05405, USA. sbarritt@g.harvard.edu.
³ Department of Biomedical and Health Sciences, University of Vermont, Burlington, VT 05405, USA. sbarritt@g.harvard.edu.
⁴ Department of Biology, University of Vermont, Burlington, VT 05405, USA. marion.weir@cellsignal.com.
⁵ Department of Biomedical and Health Sciences, University of Vermont, Burlington, VT 05405, USA. jacmann@umich.edu.
⁶ Department of Biomedical and Health Sciences, University of Vermont, Burlington, VT 05405, USA. Tyler.Hogan@uvm.edu.
⁷ Department of Biology, University of Vermont, Burlington, VT 05405, USA. bballif@uvm.edu.
⁸ Department of Biomedical and Health Sciences, University of Vermont, Burlington, VT 05405, USA. paula.deming@med.uvm.edu.

Abstract

The cAMP-dependent protein kinase A (PKA) is a serine/threonine kinase involved in many fundamental cellular processes, including migration and proliferation. Recently, we found that the Src family kinase Fyn phosphorylates the catalytic subunit of PKA (PKA-C) at Y69, thereby increasing PKA kinase activity. We also showed that Fyn induced the phosphorylation of cellular proteins within the PKA preferred target motif. This led to the hypothesis that Fyn could affect proteins in complex with PKA. To test this, we employed a quantitative mass spectrometry approach to identify Fyn-dependent binding partners in complex with PKA-C. We found Fyn enhanced the binding of PKA-C to several cytoskeletal regulators that localize to the centrosome and Golgi apparatus. Three of these Fyn-induced PKA interactors, AKAP9, PDE4DIP, and CDK5RAP2, were validated biochemically and were shown to exist in complex with Fyn and PKA in a glioblastoma cell line. Intriguingly, the complexes formed between PKA-C and these known AKAPs were dependent upon Fyn catalytic activity and expression levels. In addition, we identified Fyn-regulated phosphorylation sites on proteins in complex with PKA-C. We also identified and biochemically validated a novel PKA-C interactor, LARP4, which complexed with PKA in the absence of Fyn. These results demonstrate the ability of Fyn to influence the docking of PKA to specific cellular scaffolds and suggest that Fyn may affect the downstream substrates targeted by PKA.

Keywords: AKAPs; Fyn; Golgi apparatus; LARP4; SILAC; binding partners; centrosome; mass spectrometry; protein kinase A.

Grants and funding

8P20GM103449/NH/NIH HHS/United States