Co-metabolic enhancement of 1H-1,2,4-triazole biodegradation through nitrification

Abstract

Due to highly recalcitrant nature of 1H-1,2,4-triazole (TZ), the conventional biological process is quite ineffective for TZ removal from wastewater. In this study, co-metabolic enhancement of TZ biodegradation through nitrification was investigated in an activated sludge reactor. The link between enhanced TZ degradation and nitrification was established through highly efficient removal of TZ, TOC as well as dissolved organic matter with the supplement of NH₄⁺. A new co-metabolic degradation pathway of TZ was proposed based on the identification of five co-metabolic intermediates, including 2,4-dihydro-[1,2,4]triazol-3-one and [1,2,4]triazolidine-3,5-dione. High-throughput sequencing analysis suggested the significant improvement of microbial community in the co-metabolic system in terms of richness, abundance and uniformity. Functional species related to nitrification and biodegradation was enriched with the supplement of NH₄⁺, confirming the key role of nitrification. This study demonstrated that nitrification-assisted co-metabolism had a promising potential for the removal of recalcitrant contaminants such as TZ from wastewater.

Keywords: 1H-1,2,4-triazole; Bioaugmentation; Biodegradation; Co-metabolism; Nitrification.