Hybrid electrochemical sensor platform for capsaicin determination using coarsely stepped cyclic squarewave voltammetry

Biosens Bioelectron. 2019 Apr 1:130:374-381. doi: 10.1016/j.bios.2018.09.036. Epub 2018 Sep 14.

Abstract

A small, standalone electrochemical hybrid sensor platform, combining flexible electronics and screen-printed electrodes, is demonstrated in the determination of capsaicin through adsorptive stripping voltammetry. The sensing scheme was simplified to be compatible with a low-cost device. The simplification involved eliminating the need for additional modification of the electrode and employing a coarsely stepped squarewave voltammetry, a technique which is applicable with less sophisticated instrumentation. This architecture was found to be suitable for concentrations up to at least 5000 µM with a detection limit of 1.98 µM. The screen-printed carbon graphite electrodes were made reusable through an ethanol rinsing protocol. The effect of ethanol/buffer volumetric ratio in the test sample was shown to greatly influence the analytical data, and a fixed 10% (v/v) was chosen as a compromise between signal-to-noise ratio and not exceeding the solubility limit of the desired upper range.

Keywords: Capsaicin; Chili hotness; Electrochemical biosensors; Hybrid electronics; Screen-printed electrodes.

MeSH terms

  • Adsorption
  • Biosensing Techniques*
  • Capsaicin / chemistry
  • Capsaicin / isolation & purification*
  • Carbon / chemistry
  • Electrochemical Techniques*
  • Electrodes

Substances

  • Carbon
  • Capsaicin