Lipid profile perturbations in the plasma and lungs of mice with LPS-induced acute lung injury revealed by UHPLC-ESI-Q Exactive HF MS analysis

J Pharm Biomed Anal. 2019 Jan 5:162:242-248. doi: 10.1016/j.jpba.2018.09.037. Epub 2018 Sep 19.

Abstract

An UHPLC-ESI-Q Exactive HF MS-based lipidomics method was successfully applied to profile various lipids from the plasma and lungs of mice intranasally challenged with lipopolysaccaride (LPS). Response trends of lipids to LPS were graphically represented by variable importance in projection (VIP) plot, heat map, and bar plot. As a result, 77 differential lipids in the lung and 13 differential lipids in the plasma were identified by comparison between healthy and LPS- induced mice. These results revealed the correlation between inflammation and lipids metabolism. The differentially regulated lipids could also be potentially used as biomarkers for inflammation.

Keywords: Acute lung injury; Inflammation; Lipidomics; Lipopolysaccharide; UHPLC-Q Exactive™ HF MS.

MeSH terms

  • Acute Lung Injury / blood*
  • Acute Lung Injury / chemically induced
  • Acute Lung Injury / pathology
  • Animals
  • Biomarkers / blood
  • Chromatography, High Pressure Liquid*
  • Disease Models, Animal
  • Inflammation Mediators / blood*
  • Lipids / blood*
  • Lipopolysaccharides*
  • Lung / metabolism*
  • Lung / pathology
  • Male
  • Metabolomics / methods*
  • Mice, Inbred BALB C
  • Spectrometry, Mass, Electrospray Ionization*

Substances

  • Biomarkers
  • Inflammation Mediators
  • Lipids
  • Lipopolysaccharides