Identifying the geographical origin of protected sea cucumbers (Apostichopus japonicus) in China using random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR)

Zhenyu Yun; Zhao Sun; Haiyan Xu; Zhihong Sun; Yao Zhang; Zhen Liu

doi:10.1007/s10068-017-0048-8

Identifying the geographical origin of protected sea cucumbers (Apostichopus japonicus) in China using random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR)

Food Sci Biotechnol. 2017 Apr 30;26(2):357-362. doi: 10.1007/s10068-017-0048-8. eCollection 2017.

Authors

Zhenyu Yun¹, Zhao Sun¹, Haiyan Xu², Zhihong Sun², Yao Zhang¹, Zhen Liu¹

Affiliations

¹ 1Sub-Institute of Food and Agriculture Standardization, China National Institute of Standardization, Beijing, 100191 China.
² 2College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, 010018 China.

Abstract

Dalian sea cucumber, Yantai sea cucumber, and Weihai sea cucumber, which belong to Apostichopus japonicus, are protected as geographical indications in China based on their high nutritional values and medical propertys. The 26 samples, including Dalian sea cucumbers (9 samples) in Liaoning province, Yantai sea cucumbers (9 samples), and Weihai sea cucumbers (8 samples) in Shandong province, were individually collected from the designated geographical sea areas and the genetic relationships and DNA polymorphisms were evaluated by random amplified polymorphic DNA technology and gene segments sequencing. The RAPD dendrogram showed that the genetic diversity of the three types of sea cucumbers was rich. The neighbor-joining tree shows that the genetic relationship of the samples from the adjacent sea areas is closer. It demonstrates that the gene characteristics of sea cucumbers from different sea areas were obvious and the genetic diversity analysis by RAPD-PCR can be used as a rapid method for geographical discrimination.

Keywords: genetic diversity; geographical origin; random amplified polymorphic DNA; sea cucumber.