Antimicrobial activity of a novel furan fatty acid, 7,10-epoxyoctadeca-7,9-dienoic acid against methicillin-resistant Staphylococcus aureus

Chakradhar Dasagrandhi; Joel B Ellamar; Young Soon Kim; Hak-Ryul Kim

doi:10.1007/s10068-016-0257-6

Antimicrobial activity of a novel furan fatty acid, 7,10-epoxyoctadeca-7,9-dienoic acid against methicillin-resistant Staphylococcus aureus

Food Sci Biotechnol. 2016 Dec 31;25(6):1671-1675. doi: 10.1007/s10068-016-0257-6. eCollection 2016.

Authors

Chakradhar Dasagrandhi¹, Joel B Ellamar¹, Young Soon Kim², Hak-Ryul Kim^{1

3}

Affiliations

¹ 1School of Food Science and Biotechnology, Kyungpook National University, Daegu, 41566 Korea.
² 2Department of Food Science and Nutrition, Korea University, Seoul, 02841 Korea.
³ 3Institute of Agricultural Science & Technology, Kyungpook National University, Daegu, 41566 Korea.

Abstract

We analyzed the antimicrobial potential of a novel furan fatty acid, 7,10-epoxyoctadeca-7,9-dienoic acid (7,10-EODA) against methicillin-resistant and -sensitive S. aureus (MRSA and MSSA). The anti-staphylococcal activity of 7,10-EODA and its consequences on cell physiology was determined by disc diffusion, broth microdilution, and flow cytometry. Anti-virulence activity of 7,10-EODA was evaluated by bioassays. 7,10-EODA was anti-staphylococcal with minimum inhibitory concentration (MIC) range of 125-250 mg/L. 7,10-EODA exhibited a dose response and inhibited MRSA 01ST001 by 90.5% and ATCC 29213 (MSSA) by 85.3% at 125 mg/L. MIC of 7,10-EODA permeabilized >95 % of MRSA 01ST001 cells to small molecules. Sublethal dose of 7,10-EODA was non-toxic but markedly reduced the hemolytic, coagulase, and autolytic activities of MRSA and MSSA at 15.6 mg/L. The results provide a lead for the utilization of natural furan fatty acids as novel anti-MRSA agents.

Keywords: 7,10-epoxyoctadeca-7,9-dienoic acid; antimicrobial lipids; furan fatty acids; methicillin-resistant Staphylococcus aureus; virulence inhibition.