A dual-emission and mitochondria-targeted fluorescent probe for rapid detection of SO2 derivatives and its imaging in living cells

Abstract

A novel mitochondria-targeted fluorescent probe for sensing SO₂ derivatives was constructed by integrating phenanthroimidazole and cyanine moieties. Excited by different channels, this probe exhibited unique dual-channel fluorescence-enhanced emissions and colorimetric response to HSO₃^- quickly (less than 20 s). The sensing process was confirmed to undergo nucleophilic addition of HSO₃^- to C˭C double bond in the probe Mito-PTB via NMR and HRMS analyses. Furthermore, fluorescence co-localization studies demonstrated that the probe Mito-PTB was a mitochondria-targetable fluorescent probe for SO₂ derivatives with good cell membrane permeability. Thus, probe Mito-PTB has the potential applications to explore the role played by SO₂ derivatives in biology.

Keywords: Colorimetric; Dual-emission; Fluorescent probe; Mitochondria; SO(2) derivatives.