Development of Aurantiochytrium limacinum SR21 cultivation using salt-rich waste feedstock for docosahexaenoic acid production and application of natural colourant in food product

Hui Yi Leong; Chien-An Su; Bo-Sheng Lee; John Chi-Wei Lan; Chung Lim Law; Jo-Shu Chang; Pau Loke Show

doi:10.1016/j.biortech.2018.09.093

Development of Aurantiochytrium limacinum SR21 cultivation using salt-rich waste feedstock for docosahexaenoic acid production and application of natural colourant in food product

Bioresour Technol. 2019 Jan:271:30-36. doi: 10.1016/j.biortech.2018.09.093. Epub 2018 Sep 19.

Authors

Hui Yi Leong¹, Chien-An Su², Bo-Sheng Lee², John Chi-Wei Lan², Chung Lim Law³, Jo-Shu Chang⁴, Pau Loke Show⁵

Affiliations

¹ Bioseparation Research Group, Department of Chemical and Environmental Engineering, Faculty of Engineering, University of Nottingham Malaysia Campus, Jalan Broga, 43500 Semenyih, Selangor Darul Ehsan, Malaysia; Biorefinery and Bioprocess Engineering Laboratory, Department of Chemical Engineering and Materials Science, Yuan Ze University, No. 135, Yuan-Tung Road, Chungli, Taoyuan 320, Taiwan.
² Biorefinery and Bioprocess Engineering Laboratory, Department of Chemical Engineering and Materials Science, Yuan Ze University, No. 135, Yuan-Tung Road, Chungli, Taoyuan 320, Taiwan.
³ Department of Chemical and Environmental Engineering, Faculty of Engineering, University of Nottingham Malaysia Campus, Jalan Broga, 43500 Semenyih, Selangor Darul Ehsan, Malaysia.
⁴ Department of Chemical Engineering, National Cheng Kung University, Tainan 701, Taiwan; Research Center for Energy Technology and Strategy, National Cheng Kung University, Tainan 701, Taiwan; Research Center for Circular Economy, National Cheng Kung University, Tainan 701, Taiwan.
⁵ Bioseparation Research Group, Department of Chemical and Environmental Engineering, Faculty of Engineering, University of Nottingham Malaysia Campus, Jalan Broga, 43500 Semenyih, Selangor Darul Ehsan, Malaysia. Electronic address: PauLoke.Show@nottingham.edu.my.

PMID: 30261334
DOI: 10.1016/j.biortech.2018.09.093

Abstract

Microalgae biorefinery is presently receiving a lot of attention as driven by its production of high value-added products. In this study, an oleaginous microalga Aurantiochytrium limacinum SR21 was cultured for docosahexaenoic acid (DHA) production using 20% (w/v) of K₂HPO₄-waste feedstock to replace 0.005% (w/v) of KH₂PO₄ in the flask culture. DHA is an essential nutrient for human's brain functionalities. Collectively, the K₂HPO₄-waste feedstock with working concentration of 0.005% (w/v) in the cultivation prompted a higher lipid content (8.29%) and DHA production (128.81 mg.L^-1). Moreover, natural plant pigment products containing stabilised betacyanins were utilised as natural red colourants for hard candy production. This study develops microalgal cultivation using salt-rich waste feedstock for a higher lipid and DHA content as well as application of natural colouring agents in food products.

Keywords: Aurantiochytrium limacinum SR21; Docosahexaenoic acid; Microalgae biorefinery; Natural colourant; Salt-rich waste feedstock.

MeSH terms

Color
Docosahexaenoic Acids / biosynthesis*
Food*
Sodium Chloride / pharmacology*
Stramenopiles / drug effects
Stramenopiles / metabolism*

Substances

Docosahexaenoic Acids
Sodium Chloride