Harnessing CRISPR/Cas 9 System for manipulation of DNA virus genome

Saeedeh Ebrahimi; Ali Teimoori; Hashem Khanbabaei; Maryam Tabasi

doi:10.1002/rmv.2009

Harnessing CRISPR/Cas 9 System for manipulation of DNA virus genome

Rev Med Virol. 2019 Jan;29(1):e2009. doi: 10.1002/rmv.2009. Epub 2018 Sep 27.

Authors

Saeedeh Ebrahimi^{1

2}, Ali Teimoori^{1

2}, Hashem Khanbabaei³, Maryam Tabasi²

Affiliations

¹ Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
² Department of Virology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
³ Medical Physics Department, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

PMID: 30260068
DOI: 10.1002/rmv.2009

Abstract

The recent development of the Clustered Regularly Interspaced Palindromic Repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) system, a genome editing system, has many potential applications in virology. The possibility of introducing site specific breaks has provided new possibilities to precisely manipulate viral genomics. Here, we provide diagrams to summarize the steps involved in the process. We also systematically review recent applications of the CRISPR/Cas9 system for manipulation of DNA virus genomics and discuss the therapeutic potential of the system to treat viral diseases.

Keywords: CAS9; CRISPR-CAS system; DNA Viruses; Gene Editing.

Publication types

Review

MeSH terms

CRISPR-Associated Protein 9 / metabolism*
Clustered Regularly Interspaced Short Palindromic Repeats*
DNA Viruses / genetics*
Gene Editing / methods*
Recombination, Genetic

Substances

CRISPR-Associated Protein 9