Overview of Biochemical Assays in Lipidic Cubic Phase

Zhu Lan; Ming-Yue Lee; Eugene Chun; Bin Liu; Wei Liu

doi:10.1016/j.tibs.2018.08.008

Overview of Biochemical Assays in Lipidic Cubic Phase

Trends Biochem Sci. 2019 Apr;44(4):295-299. doi: 10.1016/j.tibs.2018.08.008. Epub 2018 Sep 19.

Authors

Zhu Lan¹, Ming-Yue Lee¹, Eugene Chun¹, Bin Liu¹, Wei Liu²

Affiliations

¹ School of Molecular Sciences, and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287-1604, USA.
² School of Molecular Sciences, and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287-1604, USA. Electronic address: w.liu@asu.edu.

PMID: 30243833
DOI: 10.1016/j.tibs.2018.08.008

Abstract

The development of novel biochemical methods to efficiently characterize membrane protein (MP) properties in lipidic cubic phase (LCP) is important for studying complicated MPs and their multimeric complexes. Here, we summarize recent LCP-related assays and provide an outlook on their applications in structure and function studies of MPs.

Keywords: LCP; crystallization; functional assay; membrane protein; protein stability.

Publication types

Review

MeSH terms

Crystallography, X-Ray
Lipids / chemistry*
Membrane Proteins / chemistry
Membrane Proteins / metabolism
Models, Molecular

Substances

Lipids
Membrane Proteins