Degradation of carboxymethylcellulose using ultrasound and β-glucanase: Pathways, kinetics and hydrolysates' properties

Furong Hou; Lihua Fan; Xiaobin Ma; Danli Wang; Wenjun Wang; Tian Ding; Xingqian Ye; Donghong Liu

doi:10.1016/j.carbpol.2018.07.092

Degradation of carboxymethylcellulose using ultrasound and β-glucanase: Pathways, kinetics and hydrolysates' properties

Carbohydr Polym. 2018 Dec 1:201:514-521. doi: 10.1016/j.carbpol.2018.07.092. Epub 2018 Jul 31.

Authors

Furong Hou¹, Lihua Fan², Xiaobin Ma³, Danli Wang⁴, Wenjun Wang⁵, Tian Ding⁶, Xingqian Ye⁷, Donghong Liu⁸

Affiliations

¹ College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China. Electronic address: houfurong2010@163.com.
² College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China. Electronic address: afanlihua@163.com.
³ College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China. Electronic address: xbma@zju.edu.cn.
⁴ College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China. Electronic address: danliwang@zju.edu.cn.
⁵ College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China. Electronic address: wangwj@zju.edu.cn.
⁶ College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China; Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang R&D Center for Food Technology and Equipment, Hangzhou 310058, China. Electronic address: tding@zju.edu.cn.
⁷ College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China; Fuli Institute of Food Science, Zhejiang University, Hangzhou 310058, China; Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang R&D Center for Food Technology and Equipment, Hangzhou 310058, China. Electronic address: psu@zju.edu.cn.
⁸ College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China; Fuli Institute of Food Science, Zhejiang University, Hangzhou 310058, China; Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang R&D Center for Food Technology and Equipment, Hangzhou 310058, China. Electronic address: dhliu@zju.edu.cn.

PMID: 30241848
DOI: 10.1016/j.carbpol.2018.07.092

Abstract

In order to provide an efficient way to degrade carboxymethylcellulose (CMC), three pathways were investigated: enzymolysis, combination of ultrasound pretreatment and enzymolysis, and sonoenzymolysis. Effects of these treatments on enzymatic kinetics, degradation kinetics and properties of degraded CMC were investigated. The degradation degree of CMC was increased by 18.90% and 35.73% with ultrasound pretreatment (at an intensity of 24 W/mL for 30 min) and sonoenzymolysis (at an intensity of 9 W/mL for 50 min), compared with that obtained under the traditional enzymolysis. Analysis of kinetics demonstrated that ultrasound, both pretreatment and combined with β-glucanase, could accelerate CMC degradation. Measurements of rheological properties, molecular weight and structures of CMC hydrolysates revealed that ultrasound broke the glycosidic bond of CMC chains without changing its primary structure. The sonoenzymolysis process was the most efficient method to degrade CMC, with potential to provide a way to obtain CMC with lowest molecular weight or viscosity.

Keywords: Carboxymethylcellulose; Degradation; Ultrasound; β-Glucanase.

MeSH terms

Carboxymethylcellulose Sodium / chemistry*
Fungal Proteins / chemistry*
Hydrolysis
Trichoderma / enzymology*
Ultrasonic Waves*
beta-Glucosidase / chemistry*

Substances

Fungal Proteins
beta-Glucosidase
Carboxymethylcellulose Sodium