Abstract
A series of macrocyclic pyrido-pentapeptide candidates 2⁻6 were synthesized by using N,N-bis-[1-carboxy-2-(benzyl)]-2,6-(diaminocarbonyl)pyridine 1a,b as starting material. Structures of the newly synthesized compounds were established by IR, ¹H and 13C-NMR, and MS spectral data and elemental analysis. The in-vitro cytotoxicity activity was investigated for all compounds against MCF-7 and HepG-2 cell lines and the majority of the compounds showed potent anticancer activity against the tested cell lines in comparison with the reference drugs. Out of the macrocyclic pyrido-pentapeptide based compounds, 5c showed encouraging inhibitory activity on MCF-7 and HepG-2 cell lines with IC50 values 9.41 ± 1.25 and 7.53 ± 1.33 μM, respectively. Interestingly, 5c also demonstrated multitarget profile and excellent inhibitory activity towards VEGFR-2, CDK-2 and PDGFRβ kinases. Furthermore, molecular modeling studies of the compound 5c revealed its possible binding modes into the active sites of those kinases.
Keywords:
in vitro anticancer activity; macrocyclic pentapeptides; molecular modeling studies; multitarget.
MeSH terms
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Antineoplastic Agents / chemistry*
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Antineoplastic Agents / pharmacology
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Cell Proliferation / drug effects
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Cyclin-Dependent Kinase 2 / antagonists & inhibitors
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Cyclin-Dependent Kinase 2 / genetics
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Drug Screening Assays, Antitumor
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Gene Expression Regulation, Neoplastic / drug effects
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Hep G2 Cells
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Humans
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MCF-7 Cells
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Macrocyclic Compounds / chemistry
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Macrocyclic Compounds / pharmacology
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Molecular Docking Simulation
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Molecular Structure
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Neoplasms / drug therapy*
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Neoplasms / genetics
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Neoplasms / pathology
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Peptides / chemistry*
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Peptides / pharmacology
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Protein Kinase Inhibitors / chemistry*
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Protein Kinase Inhibitors / pharmacology
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Receptor, Platelet-Derived Growth Factor beta / antagonists & inhibitors
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Receptor, Platelet-Derived Growth Factor beta / genetics
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Structure-Activity Relationship
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Vascular Endothelial Growth Factor Receptor-2 / antagonists & inhibitors
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Vascular Endothelial Growth Factor Receptor-2 / genetics
Substances
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Antineoplastic Agents
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Macrocyclic Compounds
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Peptides
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Protein Kinase Inhibitors
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Receptor, Platelet-Derived Growth Factor beta
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Vascular Endothelial Growth Factor Receptor-2
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Cyclin-Dependent Kinase 2