In-Vitro cytotoxicity, antibacterial, and UV protection properties of the biosynthesized Zinc oxide nanoparticles for medical textile applications

Amr Fouda; Saad El-Din Hassan; Salem S Salem; Tharwat I Shaheen

doi:10.1016/j.micpath.2018.09.030

In-Vitro cytotoxicity, antibacterial, and UV protection properties of the biosynthesized Zinc oxide nanoparticles for medical textile applications

Microb Pathog. 2018 Dec:125:252-261. doi: 10.1016/j.micpath.2018.09.030. Epub 2018 Sep 18.

Authors

Amr Fouda¹, Saad El-Din Hassan¹, Salem S Salem¹, Tharwat I Shaheen²

Affiliations

¹ Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, Nasr City, Cairo, 11884, Egypt.
² National Research Centre (Scopus affiliation ID 60014618), TIRD, El-Behouth St. (former El-Tahrir str.), Dokki, P.O. 12622, Giza, Egypt; Biopolymers Interact & Assemblages Research Unit, INRA, F-44316, Nantes, France. Electronic address: Shaheen_chem@yahoo.com.

PMID: 30240818
DOI: 10.1016/j.micpath.2018.09.030

Abstract

Nowadays, medical textiles have become the most essential and developing part in human healthcare sector. This work was undertaken with a view to harness the bio-active macromolecules secreted by fungi e.g. proteins and enzymes in bio-synthesis of ZnO nanoparticles for multifunctional textiles such as antibacterial activity and UV protection with considering the cytotoxicity limitation. Herein, the isolated fungus, Aspergillus terreus, was allowed to produce proteins which has affinity to cape ZnO-NPs. Various factors affecting the behavior of the secreted proteins on the formed nanoparticles were investigated. Thorough characterizations of the protein capped ZnO-NPs were performed by the using of UV-Visible spectroscopy, transmission electron microscope (TEM) Fourier Transform-Infra Red (FT-IR) spectroscopy, X-ray diffraction (XRD) analysis and Dynamic light scattering analysis (DLS). Prior treatment of cotton fabrics with ZnO-NPs, the cytotoxicity of the protein capped ZnO-NPs was examined. After that, the antibacterial activity of the ZnO-NPs before and after treating of cotton fabrics, besides, the UV-protection (UPF) properties were investigated. Results obviously demonstrated the ability of the bio-secreted protein to cape and reduce ZnO to spherical ZnO-NPs with particle size lied around 10-45 nm, as indicated form UV-vis., spectra TEM, Zeta sizer, FTIR and XRD. Regarding to the results of cytotoxicity, the treatment of the cotton fabrics with ZnO-NPs were performed at safe dose (20 ppm). At this dose, ZnO-NPs loaded samples exhibited reasonable antibacterial activity against both Gram positive and Gram negative bacteria; besides, good UV-protection with reasonable increase in UVA and UVB blocking values. Indeed, nanotechnology based microbiological active molecules opens up new opportunities for us to explore novel applications in terms of green technology.

Keywords: Antibacterial; Biosynthesis; Cytotoxicity; Medical textile; UV protection; Zinc oxide nanoparticles.

MeSH terms

Animals
Anti-Bacterial Agents / pharmacology*
Aspergillus / metabolism*
Cell Line
Cell Survival / drug effects
Disease Transmission, Infectious / prevention & control*
Epithelial Cells / drug effects
Epithelial Cells / physiology
Gram-Negative Bacteria / drug effects
Gram-Positive Bacteria / drug effects
Humans
Metal Nanoparticles / chemistry
Metal Nanoparticles / toxicity
Metal Nanoparticles / ultrastructure
Radiation-Protective Agents / pharmacology*
Textiles*
Ultraviolet Rays
Zinc Oxide / chemistry
Zinc Oxide / pharmacology*
Zinc Oxide / toxicity*

Substances

Anti-Bacterial Agents
Radiation-Protective Agents
Zinc Oxide