Tannic Acid Cross-linked Polysaccharide-Based Multifunctional Hemostatic Microparticles for the Regulation of Rapid Wound Healing

Macromol Biosci. 2018 Nov;18(11):e1800209. doi: 10.1002/mabi.201800209. Epub 2018 Sep 20.

Abstract

Hemostatic microparticles (HMs) have been widely used in surgery. To improve the comprehensive performance of HMs, multifunctional HMs named HM15 and HM15 ' are prepared from starch, carboxymethyl chitosan, hyaluronic acid, and tannic acid. Herein, tannic acid is used as an effective cross-linker. A 3D network structure for cell growth and wound repair can be formed by secondary cross-linking. Through synergistic effect of these natural materials, the process of wound healing can be regulated controllably. HM15 and HM15 ' have the ability of rapid hemostasis. Moreover, HM15 ' shows excellent properties in antibacteria and wound healing acceleration. Blood clotting time treated with different HMs is shortened obviously from 436.8 s to 126 s. Compared with Celox, HM15 and HM15 ' exhibited better broad spectrum antibacterial activity against both Escherichia coli and Staphylococcus aureus. Notably, the wound can be repaired rapidly by HM15 ' in 14 days. These multifunctional HMs might have an important prospect in clinical application.

Keywords: antibacteria; hemostasis; microparticle; multifunctional; wound healing.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Bacterial Agents* / chemistry
  • Anti-Bacterial Agents* / pharmacology
  • Cell Line
  • Cross-Linking Reagents / chemistry*
  • Escherichia coli / growth & development*
  • Hemostatics* / chemistry
  • Hemostatics* / pharmacology
  • Humans
  • Mice
  • Polysaccharides* / chemistry
  • Polysaccharides* / pharmacology
  • Staphylococcus aureus / growth & development*
  • Tannins / chemistry*
  • Wound Healing / drug effects*

Substances

  • Anti-Bacterial Agents
  • Cross-Linking Reagents
  • Hemostatics
  • Polysaccharides
  • Tannins