Effect of Spanish-style processing steps and inoculation with Lactobacillus pentosus starter culture on the volatile composition of cv. Manzanilla green olives

Food Chem. 2019 Jan 15:271:543-549. doi: 10.1016/j.foodchem.2018.07.166. Epub 2018 Jul 25.

Abstract

The effects of the main steps of Spanish-style processing (alkaline treatment and fermentation) on the volatile composition of cv. Manzanilla green olives were studied. Both spontaneous and controlled fermentations were considered. In the latter case, a Lactobacillus pentosus strain from green olive fermentation brine was used as starter culture. The volatile profile was determined by headspace solid-phase microextraction (HS-SPME) combined with gas chromatography-mass spectrometry (GC-MS). Most of the volatile compounds detected in fresh olives decreased or were undetected after alkaline treatment, while several compounds (mostly acetic acid, 2-methylbutanoic acid, and ethanol) were formed as a result of this treatment. Over 50 new volatile components, mostly esters and phenols, appeared as a result of fermentation. The most outstanding finding was a considerable increase in 4-ethyl phenol (almost 100-fold increase) in inoculated olives compared to the uninoculated product. However, a sensory panel did not find significant differences in odor perception.

Keywords: Lactobacillus pentosus; Manzanilla cultivar; Processing; Table olives; Volatile composition.

MeSH terms

  • Fermentation
  • Food Handling / methods*
  • Food Microbiology*
  • Hydrogen-Ion Concentration
  • Lactobacillus
  • Lactobacillus pentosus / physiology*
  • Oils, Volatile / analysis*
  • Olea / chemistry
  • Olea / microbiology*

Substances

  • Oils, Volatile