Sirt1 protects neural stem cells from apoptosis by decreasing acetylation of histone 3K9

Chongdong Jian; Cuihua Zou; Ning Xu; Guoying Chen; Donghua Zou

doi:10.2147/SCCAA.S173852

Sirt1 protects neural stem cells from apoptosis by decreasing acetylation of histone 3K9

Stem Cells Cloning. 2018 Sep 7:11:39-41. doi: 10.2147/SCCAA.S173852. eCollection 2018.

Authors

Chongdong Jian¹, Cuihua Zou¹, Ning Xu², Guoying Chen², Donghua Zou²

Affiliations

¹ Youjiang Medical University for Nationalities, Baise, Guangxi 533000, People's Republic of China.
² Department of Neurology, The Fifth Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530021, People's Republic of China, danvor0922@hotmail.com.

Abstract

Objective: To explore the role and mechanism of Sirt1 in protecting neural stem cells (NSCs) from apoptosis.

Materials and methods: Transfection was used to overexpress Sirt1 in rat NSCs. The effect of Sirt1 overexpression on camptothecin-induced apoptosis of NSCs was evaluated. Western blotting was used to examine the expression of Sirt1, cleaved caspase-3, and acetylated histone 3K9.

Results: Overexpression of Sirt1 in NSCs decreased the cleavage of caspase-3 and acetylation of histone 3K9.

Conclusion: Sirt1 may protect NSCs from apoptosis by decreasing the acetylation of histone 3 on K9.

Keywords: Sirt1; acetylated histone 3K9; apoptosis; caspase-3; neural stem cells.