Facile Photochemical Modification of Silk Protein-Based Biomaterials

John G Hardy; Annabelle Bertin; Jose Guillermo Torres-Rendon; Aldo Leal-Egaña; Martin Humenik; Felix Bauer; Andreas Walther; Helmut Cölfen; Helmut Schlaad; Thomas R Scheibel

doi:10.1002/mabi.201800216

Facile Photochemical Modification of Silk Protein-Based Biomaterials

Macromol Biosci. 2018 Nov;18(11):e1800216. doi: 10.1002/mabi.201800216. Epub 2018 Sep 19.

Authors

John G Hardy^{1

2

3}, Annabelle Bertin^{4

5}, Jose Guillermo Torres-Rendon⁶, Aldo Leal-Egaña^{1

7}, Martin Humenik¹, Felix Bauer¹, Andreas Walther^{6

8

9

10}, Helmut Cölfen¹¹, Helmut Schlaad¹², Thomas R Scheibel^{1

13

14

15

16

17}

Affiliations

¹ Biomaterials, Faculty of Engineering Science, University of Bayreuth, Universitätsstraße 30, 95447, Bayreuth, Germany.
² Department of Chemistry, Lancaster University, Lancaster, LA1 4YB, UK.
³ Materials Science Institute, Lancaster University, Lancaster, LA1 4YB, UK.
⁴ German Federal Institute for Materials Research and Testing (BAM), Unter den Eichen 87, 12205, Berlin, Germany.
⁵ Institute of Chemistry and Biochemistry, Free University of Berlin, Takustraße 3, 14195, Berlin, Germany.
⁶ DWI Leibniz Institute for Interactive Materials, Forckenbeckstraße 50, 52056, Aachen, Germany.
⁷ Institute of Biomaterials, University of Erlangen-Nuremberg, Ulrich-Schalk-Straße 3, 91056, Erlangen, Germany.
⁸ Institute for Macromolecular Chemistry, University of Freiburg, Stefan-Meier-Straße 31, 79104, Freiburg, Germany.
⁹ Freiburg Materials Research Center, University of Freiburg, Stefan-Meier-Straße 21, 79104, Freiburg, Germany.
¹⁰ Freiburg Center for Interactive Materials and Bioinspired Technologies, University of Freiburg, Georges-Köhler-Allee 105, 79110, Freiburg, Germany.
¹¹ Physical Chemistry Group, Department of Chemistry, University of Konstanz, Universitätsstraße 10, 78457, Konstanz, Germany.
¹² Institute of Chemistry, University of Potsdam, Karl-Liebknecht-Straße 24-25, 14476, Potsdam, Germany.
¹³ Bayreuther Zentrum für Kolloide und Grenzflächen (BZKG), Universität Bayreuth, Universitätsstraße 30, 95440, Bayreuth, Germany.
¹⁴ Bayerisches Polymerinstitut (BPI), Universität Bayreuth, Universitätsstraße 30, 95440, Bayreuth, Germany.
¹⁵ Bayreuther Zentrum für Bio-Makromoleküle (Bio-Mac), Universität Bayreuth, Universitätsstraße 30, 95440, Bayreuth, Germany.
¹⁶ Bayreuther Zentrum für Molekulare Biowissenschaften (BZMB), Universität Bayreuth, Universitätsstraße 30, 95440, Bayreuth, Germany.
¹⁷ Bayreuther Materialzentrum (BayMAT), Universität Bayreuth, Universitätsstraße 30, 95440, Bayreuth, Germany.

PMID: 30230222
DOI: 10.1002/mabi.201800216

Abstract

Silk protein-based materials show promise for application as biomaterials for tissue engineering. The simple and rapid photochemical modification of silk protein-based materials composed of either Bombyx mori silkworm silk or engineered spider silk proteins (eADF4(C16)) is reported. Radicals formed on the silk-based materials initiate the polymerization of monomers (acrylic acid, methacrylic acid, or allylamine) which functionalize the surface of the silk materials with poly(acrylic acid) (PAA), poly(methacrylic acid) (PMAA), or poly(allylamine) (PAAm). To demonstrate potential applications of this type of modification, the polymer-modified silks are mineralized. The PAA- and PMAA-functionalized silks are mineralized with calcium carbonate, whereas the PAAm-functionalized silks are mineralized with silica, both of which provide a coating on the materials that may be useful for bone tissue engineering, which will be the subject of future investigations.

Keywords: biomaterials; chemical modification; photochemistry; silkworm silk; spider silk.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acrylic Resins / chemistry*
Animals
Bombyx
Calcium Carbonate / chemistry
Coated Materials, Biocompatible* / chemical synthesis
Coated Materials, Biocompatible* / chemistry
Photochemical Processes*
Polyamines / chemistry*
Polymethacrylic Acids / chemistry*
Silk / chemistry*
Spiders

Substances

Acrylic Resins
Coated Materials, Biocompatible
Polyamines
Polymethacrylic Acids
Silk
polymethacrylic acid
polyallylamine
carbopol 940
Calcium Carbonate