HPLC-ESI-MS method for C60 fullerene mitochondrial content quantification

Anna Grebinyk; Sergii Grebinyk; Svitlana Prylutska; Uwe Ritter; Olga Matyshevska; Thomas Dandekar; Marcus Frohme

doi:10.1016/j.dib.2018.06.089

HPLC-ESI-MS method for C₆₀ fullerene mitochondrial content quantification

Data Brief. 2018 Jul 10:19:2047-2052. doi: 10.1016/j.dib.2018.06.089. eCollection 2018 Aug.

Authors

Anna Grebinyk^{1

2

3}, Sergii Grebinyk¹, Svitlana Prylutska⁴, Uwe Ritter⁵, Olga Matyshevska³, Thomas Dandekar², Marcus Frohme¹

Affiliations

¹ Division Molecular Biotechnology and Functional Genomics, Technical University of Applied Sciences Wildau, Hochschulring 1, 15745 Wildau, Germany.
² Dept. of Bioinformatics, Biocenter, University of Würzburg, Am Hubland, 97074 Würzburg, Germany.
³ Educational and Scientific Center "Institute of Biology and Medicine", Taras Shevchenko National University of Kyiv, Volodymyrska 64, 01601 Kyiv, Ukraine.
⁴ Dept. of Chemistry, Taras Shevchenko National University of Kyiv, Volodymyrska 64, 01601 Kyiv, Ukraine.
⁵ Institute of Chemistry and Biotechnology, University of Technology Ilmenau, WeimarerStraße 25 (Curiebau), 98693 Ilmenau, Germany.

Abstract

The presented dataset describes the quantification of carbon nanoparticle C₆₀ fullerene accumulated in mitochondria of human leukemic cells treated with nanostructure. Firstly, the high performance liquid chromatography-electro spray ionization-mass spectrometry (HPLC-ESI-MS) method was developed for quantitative analysis of pristine C₆₀ fullerene. Then, human leukemic cells were incubated with C₆₀ fullerene, homogenized and subjected to the differential centrifugation to retrieve mitochondrial fraction. The C₆₀ fullerene content was quantified by HPLC-ESI-MS in extracts of cellular fractions. This data article refers to the research article "C₆₀ Fullerene Accumulation in Human Leukemic Cells and Perspectives of LED-mediated Photodynamic Therapy" by Grebinyk et al. [1].