Inhibitory Activity and Docking Analysis of Antimalarial Agents from Stemona sp. toward Ferredoxin-NADP+ Reductase from Malaria Parasites

Pratiwi Pudjiastuti; Ni Nyoman T Puspaningsih; Imam Siswanto; Much Z Fanani; Yoko K Ariga; Toshiharu Hase; Satyajit D Sarker; Lutfun Nahar

doi:10.1155/2018/3469132

Inhibitory Activity and Docking Analysis of Antimalarial Agents from Stemona sp. toward Ferredoxin-NADP+ Reductase from Malaria Parasites

J Parasitol Res. 2018 Aug 26:2018:3469132. doi: 10.1155/2018/3469132. eCollection 2018.

Authors

Pratiwi Pudjiastuti¹, Ni Nyoman T Puspaningsih¹, Imam Siswanto¹, Much Z Fanani², Yoko K Ariga³, Toshiharu Hase⁴, Satyajit D Sarker⁵, Lutfun Nahar⁵

Affiliations

¹ Department of Chemistry, Faculty of Science and Technology, Airlangga University, Surabaya 60115, Indonesia.
² Laboratory of Proteomics, Institute of Tropical Disease, Airlangga University, Surabaya 60115, Indonesia.
³ Graduate School of Sciences and Technology for Innovation, Department of Agriculture, Yamaguchi University, Yamaguchi 753-8515, Japan.
⁴ Institute of Protein Research, Osaka University, 3-2 Yamadoaka, Suita-shi, Osaka 656-0871, Japan.
⁵ Medicinal Chemistry and Natural Products Research Group, School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, James Parsons Building, Byrom Street, Liverpool L3 3AF, UK.

Abstract

Ferredoxin-NADP⁺ reductases (FNRs, EC 1.18.1.2) were found in the plastids of Plasmodium and have been considered as a target for the development of new antimalarial agents. Croomine, epi-croomine, tuberostemonine, javastemonine A, and isoprotostemonine are isolated alkaloids from the roots of Stemona sp. and their inhibitory effect on FNRs from Plasmodium falciparum (PfFNR) was investigated. Croomine showed the highest level of inhibition (33.9%) of electron transfer from PfFNR to PfFd, while tuberstemonine displayed the highest level of inhibition (55.4%) of diaphorase activity of PfFNR. Docking analysis represented that croomine is located at the middle position of PfFNR and PfFd. Croomine from S. tuberosa appeared to have potential as an antimalarial agent.