This study investigates the influence of inoculated and uninoculated aeration pretreatment on nutrients and phytotoxicity of anaerobic digestion (AD) effluent. Swine manure AD effluent was inoculated with activated and anaerobic sludge, respectively. Our results show that aeration with the addition of activated sludge could reduce the phytotoxicity of AD effluent. Compared to the control treatment without any sludge addition, the addition of activated sludge resulted in a more significant reduction in the AD effluent salinity, NH4+ content, and organic matter (indicated by the chemical oxygen demand) when AD effluent was aerated for less than 8 hours. As a result, a much higher seed germination index (GI) was observed for the treatment with activated sludge addition, particularly when aerated for 4-5 hours, contributing the gas/water ratio of 20:1-30:1. On the other hand, no significant differences in the nutrient contents and GI value were observed for the control treatment and that with the addition of anaerobic sludge. Results from this study shed light on optimizing the management of AD effluent for agricultural application.