A novel pH-responsive quaternary ammonium chitosan-liposome nanoparticles for periodontal treatment

Int J Biol Macromol. 2019 May 15:129:1113-1119. doi: 10.1016/j.ijbiomac.2018.09.057. Epub 2018 Sep 12.

Abstract

The aim of this study was to evaluate the antibacterial activity and cytocompatibility of novel pH-activated nanoparticles (NPs) in vitro and in vivo. The NPs were synthesized from a quaternary ammonium chitosan, i.e., N,N,N-trimethyl chitosan, a liposome, and doxycycline (TMC-Lip-DOX NPs). The cytocompatibility of the NPs was evaluated. The TMC-Lip-DOX NPs achieved superb inhibition of free mixed bacteria and biofilm formation. They also showed excellent biocompatibility with human periodontal ligament fibroblasts. Animal experiments showed that the NPs strongly inhibited biofilm formation and prevented alveolar bone absorption in vivo. All the results indicate that the TMC-Lip-DOX NPs have good potential for use in the treatment of periodontal and other inflammatory diseases.

Keywords: Animal model; Doxycycline; Histopathology; Nanoparticles; Periodontitis; Plaque biofilm.

MeSH terms

  • Ammonium Compounds / chemistry*
  • Animals
  • Anti-Bacterial Agents / chemistry
  • Anti-Bacterial Agents / pharmacology*
  • Biofilms / drug effects
  • Chitosan / chemistry*
  • Doxycycline / chemistry
  • Doxycycline / pharmacology
  • Drug Carriers / chemistry*
  • Hydrogen-Ion Concentration
  • Liposomes / chemistry*
  • Materials Testing
  • Nanoparticles / chemistry*
  • Optical Imaging
  • Periodontal Ligament / cytology
  • Periodontal Ligament / drug effects*
  • Periodontal Ligament / microbiology
  • Porphyromonas gingivalis / drug effects
  • Prevotella intermedia / drug effects
  • Prevotella intermedia / physiology
  • Rats

Substances

  • Ammonium Compounds
  • Anti-Bacterial Agents
  • Drug Carriers
  • Liposomes
  • N-trimethyl chitosan chloride
  • Chitosan
  • Doxycycline