X-Chromosome Inactivation and Escape from X Inactivation in Mouse

Wenxiu Ma; Giancarlo Bonora; Joel B Berletch; Xinxian Deng; William S Noble; Christine M Disteche

doi:10.1007/978-1-4939-8766-5_15

X-Chromosome Inactivation and Escape from X Inactivation in Mouse

Methods Mol Biol. 2018:1861:205-219. doi: 10.1007/978-1-4939-8766-5_15.

Authors

Wenxiu Ma¹, Giancarlo Bonora², Joel B Berletch³, Xinxian Deng³, William S Noble², Christine M Disteche^{4

5}

Affiliations

¹ Department of Statistics, University of California, Riverside, Riverside, CA, USA.
² Department of Genome Sciences, University of Washington, Seattle, WA, USA.
³ Department of Pathology, University of Washington, Seattle, WA, USA.
⁴ Department of Pathology, University of Washington, Seattle, WA, USA. cdistech@u.washington.edu.
⁵ Department of Medicine, University of Washington, Seattle, WA, USA. cdistech@u.washington.edu.

Abstract

X chromosome inactivation silences one X chromosome in female mammals. However, this silencing is incomplete, and some genes escape X inactivation. We describe methods to determine the chromosome-wide X inactivation status of genes in tissues or cell lines derived from mice using a combination of skewing of X inactivation and allele-specific analyses of gene expression based on RNA-seq.

Keywords: Allelic gene expression; Escape from X inactivation; X inactivation.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Alleles*
Animals
Cell Line
Embryo, Mammalian / metabolism*
Epigenomics / methods
Female
Gene Expression Profiling / methods*
Gene Expression Regulation, Developmental
Mice
Mice, Inbred C57BL
Polymorphism, Single Nucleotide
Sequence Analysis, RNA / methods
X Chromosome Inactivation*

Abstract

Publication types

MeSH terms

Grants and funding