Development and evaluation of monoclonal antibody-based antigen capture enzyme-linked immunosorbent assay for the diagnosis of acute leptospirosis in humans

Chandika Gamage; Yomani Sarathkumara; Virajie Weerakkodi; Kanae Shiokawa; Kumiko Yoshimatsu; Jiro Arikawa

doi:10.1016/j.jim.2018.09.006

Development and evaluation of monoclonal antibody-based antigen capture enzyme-linked immunosorbent assay for the diagnosis of acute leptospirosis in humans

J Immunol Methods. 2018 Dec:463:134-136. doi: 10.1016/j.jim.2018.09.006. Epub 2018 Sep 12.

Authors

Chandika Gamage¹, Yomani Sarathkumara², Virajie Weerakkodi², Kanae Shiokawa³, Kumiko Yoshimatsu⁴, Jiro Arikawa⁴

Affiliations

¹ Department of Microbiology, Faculty of Medicine, University of Peradeniya, Sri Lanka. Electronic address: chandika@pdn.ac.lk.
² Department of Microbiology, Faculty of Medicine, University of Peradeniya, Sri Lanka.
³ Department of Biomedical Sciences, Ross University School of Veterinary Medicine, St. Kitts and Nevis, West Indies.
⁴ Laboratory of Infectious Diseases, Department of Microbiology, Graduate School of Medicine, Hokkaido University, Japan.

PMID: 30217719
DOI: 10.1016/j.jim.2018.09.006

Abstract

Monoclonal antibody (MAb)-based ELISA was developed to detect leptospiral antigens from the plasma of febrile patients. The MAb reacts specifically with pathogenic leptospires and the assay possesses excellent diagnostic test parameters compared to PCR, indicating that this new ELISA is useful for the early diagnosis of leptospirosis with low operational cost.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Antibodies, Bacterial / chemistry*
Antibodies, Monoclonal, Murine-Derived / chemistry*
Antigens, Bacterial / immunology*
Enzyme-Linked Immunosorbent Assay / methods
Humans
Leptospira interrogans / immunology*
Leptospirosis* / diagnosis
Leptospirosis* / immunology
Sensitivity and Specificity

Substances

Antibodies, Bacterial
Antibodies, Monoclonal, Murine-Derived
Antigens, Bacterial