Generation of induced pluripotent stem cell line(ZZUi006-A)from a patient with myotonic dystrophy type 1

Yanlin Wang; Zhilei Wang; Huifang Sun; Changhe Shi; Jing Yang; Yutao Liu; Han Liu; Shoutao Zhang; Li Zhang; Yuming Xu; Jin Zhang

doi:10.1016/j.scr.2018.08.013

Generation of induced pluripotent stem cell line(ZZUi006-A)from a patient with myotonic dystrophy type 1

Stem Cell Res. 2018 Oct:32:61-64. doi: 10.1016/j.scr.2018.08.013. Epub 2018 Aug 22.

Authors

Yanlin Wang¹, Zhilei Wang¹, Huifang Sun¹, Changhe Shi¹, Jing Yang¹, Yutao Liu¹, Han Liu¹, Shoutao Zhang², Li Zhang³, Yuming Xu⁴, Jin Zhang⁵

Affiliations

¹ Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
² School of life sciences, Zhengzhou University, Zhengzhou, Henan 450001, China.
³ Center for Stem Cell and Regenerative Medicine, Department of Basic Medical Sciences, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang 310058, China; Institute of Hematology, Zhejiang University, Hangzhou, Zhejiang 310058, China.
⁴ Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China. Electronic address: xuyuming@zzu.edu.cn.
⁵ Center for Stem Cell and Regenerative Medicine, Department of Basic Medical Sciences, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang 310058, China; Institute of Hematology, Zhejiang University, Hangzhou, Zhejiang 310058, China. Electronic address: zhgene@gmail.com.

PMID: 30216892
DOI: 10.1016/j.scr.2018.08.013

Abstract

Myotonic dystrophy type 1 (DM1) is a dominantly inherited neurodegenerative disease caused by a CTG repeat expansion close to the DMPK gene. The fibroblasts from a patient with DM1 were successfully converted to induced pluripotent stem cells (iPSCs), designated ZZUi006-A, by employing episomal plasmids expressing OCT3/4, SOX2, KLF4, LIN28, L-MYC. The ZZUi006-A cell line may provide a robust platform for further study of DM1 pathogenesis as well as drug testing and gene therapy research.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Differentiation / genetics
Cell Differentiation / physiology
Cell Line
Cells, Cultured
Humans
Induced Pluripotent Stem Cells / cytology*
Induced Pluripotent Stem Cells / metabolism
Karyotype
Kruppel-Like Factor 4
Male
Microsatellite Repeats / genetics
Middle Aged
Myotonic Dystrophy / metabolism*
Polymerase Chain Reaction