Removal of Microcystin-LR by a Novel Native Effective Bacterial Community Designated as YFMCD4 Isolated from Lake Taihu

Fei Yang; Jian Guo; Feiyu Huang; Isaac Yaw Massey; Ruixue Huang; Yunhui Li; Cong Wen; Ping Ding; Weiming Zeng; Geyu Liang

doi:10.3390/toxins10090363

Removal of Microcystin-LR by a Novel Native Effective Bacterial Community Designated as YFMCD4 Isolated from Lake Taihu

Toxins (Basel). 2018 Sep 8;10(9):363. doi: 10.3390/toxins10090363.

Authors

Fei Yang^{1

2

3}, Jian Guo⁴, Feiyu Huang⁵, Isaac Yaw Massey⁶, Ruixue Huang⁷, Yunhui Li⁸, Cong Wen⁹, Ping Ding¹⁰, Weiming Zeng¹¹, Geyu Liang¹²

Affiliations

¹ Department of Occupational and Environmental Health, Xiangya School of Public Health, Central South University, 110 Xiangya Road, Changsha 410078, China. phfyang@csu.edu.cn.
² Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health Southeast University, Nanjing 210009, China. phfyang@csu.edu.cn.
³ Key laboratory of Hunan Province for Water Environment and Agriculture Product Safety, Central South University, Changsha 410083, China. phfyang@csu.edu.cn.
⁴ Department of Occupational and Environmental Health, Xiangya School of Public Health, Central South University, 110 Xiangya Road, Changsha 410078, China. guojianph@csu.edu.cn.
⁵ Department of Occupational and Environmental Health, Xiangya School of Public Health, Central South University, 110 Xiangya Road, Changsha 410078, China. huangfeiyu@csu.edu.cn.
⁶ Department of Occupational and Environmental Health, Xiangya School of Public Health, Central South University, 110 Xiangya Road, Changsha 410078, China. robert@csu.edu.cn.
⁷ Department of Occupational and Environmental Health, Xiangya School of Public Health, Central South University, 110 Xiangya Road, Changsha 410078, China. zhengshl@csu.edu.cn.
⁸ Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health Southeast University, Nanjing 210009, China. yhli@seu.edu.cn.
⁹ Department of Occupational and Environmental Health, Xiangya School of Public Health, Central South University, 110 Xiangya Road, Changsha 410078, China. wencong941017@csu.edu.cn.
¹⁰ Department of Occupational and Environmental Health, Xiangya School of Public Health, Central South University, 110 Xiangya Road, Changsha 410078, China. yixp176911007@csu.edu.cn.
¹¹ Key Laboratory of Biometallurgy, Ministry of Education, School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China. hyacinth_hai@csu.edu.cn.
¹² Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health Southeast University, Nanjing 210009, China. gyliang@seu.edu.cn.

Abstract

Microcystin-LR (MC-LR) is the most toxic and frequently detected monocyclic heptapeptide hepatotoxin produced by cyanobacteria, which poses a great threat to the natural ecosystem and public health. It is very important to seek environment-friendly and cost-efficient methods to remove MC-LR in water. In this study, the MC-degrading capacities of a novel indigenous bacterial community designated as YFMCD4 and the influence of environmental factors including various temperatures, MC concentrations and pH on the MC-degrading activities were investigated utilizing high-performance liquid chromatography (HPLC). In addition, the MC-degrading mechanism of YFMCD4 was also studied using HPLC coupled with a mass spectrometry equipped with electrospray ionization interface (HPLC-ESI-MS). The data showed MC-LR was completely removed at the maximum rate of 0.5 µg/(mL·h) under the optimal condition by YFMCD4. Two pure bacterial strains Alcaligenes faecalis and Stenotrophomonas acidaminiohila were isolated from YFMCD4 degraded MC-LR at a slower rate. The MC-degrading rates of YFMCD4 were significantly affected by different temperatures, pH and MC-LR concentrations. Two intermediates of a tetrapeptide and Adda appeared in the degradation process. These results illustrate that the novel YFMCD4 is one of the highest effective MC-degrading bacterial community, which can completely remove MC-LR and possesses a significant potential to treat water bodies contaminated by MC-LR.

Keywords: Adda; bacterial community; microcystin-LR (MC-LR); remove.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alcaligenes faecalis / metabolism*
China
Chromatography, High Pressure Liquid
Hydrogen-Ion Concentration
Lakes
Marine Toxins
Microcystins / metabolism*
Spectrometry, Mass, Electrospray Ionization
Temperature
Water Microbiology
Water Pollutants / metabolism*
Water Purification / methods
Xanthomonadaceae / metabolism*

Substances

Marine Toxins
Microcystins
Water Pollutants
cyanoginosin LR